Lu Quan, Decock Cony, Zhang Xing Yao, Maraite Henri
Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, 100091 Beijing, China.
Antonie Van Leeuwenhoek. 2009 Oct;96(3):275-93. doi: 10.1007/s10482-009-9343-6. Epub 2009 Apr 30.
Dendroctonus valens is an invasive pest in coniferous forests of northern China. It was suspected of being responsible for the death of more than three million Pinus tabuliformis trees. The present study sought to identify the ophiostomatoid fungi associated with D. valens in northern China and understand the possible role of these fungi in the pine decline. On the basis of morphology, physiology, mating compatibility and phylogenetic analyses of multiple DNA sequences, seven species of ophiostomatoid fungi were isolated from and around D. valens galleries: Leptographium alethinum, Grosmannia koreana (teleomorph of L. koreanum), L. procerum, L. sinoprocerum, L. truncatum, Pesotum aureum and P. pini. All have been recorded for the first time in China. Among them, the occurrence of the dominant species L. procerum is positively linked to attack intensities of D. valens. The pathogenicity of four species (L. koreanum, L. procerum, L. sinoprocerum and L. truncatum) was tested on mature P. tabuliformis trees by stem inoculation. All inoculated strains caused significant necrotic lesions on the inner bark. However, L. koreanum and L. truncatum induced more extensive lesions than L. procerum and L. sinoprocerum. Their association with D. valens and the P. tabuliformis decline is discussed.
红脂大小蠹是中国北方针叶林的一种入侵性害虫。据怀疑，它导致了超过300万株油松树死亡。本研究旨在鉴定中国北方与红脂大小蠹相关的长喙壳类真菌，并了解这些真菌在松树衰退中可能发挥的作用。基于形态学、生理学、交配兼容性以及多个DNA序列的系统发育分析，从红脂大小蠹蛀道及其周围分离出7种长喙壳类真菌：阿勒泰细孔菌、韩国长喙壳菌（朝鲜细孔菌的有性型）、长喙细孔菌、中华长喙细孔菌、截形细孔菌、金色佩索霉和松佩索霉。所有这些真菌均为中国首次记录。其中，优势种长喙细孔菌的出现与红脂大小蠹的侵害强度呈正相关。通过树干接种对4种真菌（朝鲜细孔菌、长喙细孔菌、中华长喙细孔菌和截形细孔菌）在成熟油松树上的致病性进行了测试。所有接种菌株均在内树皮上引起了明显的坏死病变。然而，朝鲜细孔菌和截形细孔菌诱导的病变比长喙细孔菌和中华长喙细孔菌更广泛。本文讨论了它们与红脂大小蠹以及油松衰退的关系。
