Environmental Science G. Sarfatti Department, University of Siena, Siena, Italy.
Tissue Cell. 2009 Dec;41(6):443-7. doi: 10.1016/j.tice.2009.03.002. Epub 2009 Apr 29.
The ultrastructure of Vitis vinifera seeds from different archaeological sites was studied. Preservation status differed between sites. Preliminary investigations of grape seeds from Poggio Bacherina (Chianciano Terme, Siena) and Miranduolo (Chiusdino, Siena) showed collapsed or charred tegument, making this material suitable for morphometric studies only. Rapid-freeze fixation and substitution of grape seeds from Shahr-I Sokhta in Iran and via De' Castellani in Florence revealed well preserved tegument suitable for chemical and cytochemical analysis. Energy dispersive X-ray microanalysis was used to determine chemical composition. Cytochemical analysis based on fluorescent staining with DAPI suggested the presence of cytoplasm residues.
研究了来自不同考古遗址的酿酒葡萄种子的超微结构。遗址之间的保存状况不同。对来自 Poggio Bacherina（锡耶纳的 Chianciano Terme）和 Miranduolo（锡耶纳的 Chiusdino）的葡萄种子的初步研究表明，种皮已经塌陷或碳化，因此仅适合形态计量学研究。对来自伊朗 Shahr-I Sokhta 和佛罗伦萨 via De' Castellani 的葡萄种子进行快速冷冻固定和取代，揭示了保存完好的种皮，适合进行化学和细胞化学分析。能量色散 X 射线微分析用于确定化学组成。基于 DAPI 荧光染色的细胞化学分析表明存在细胞质残留物。
