Chamkh Fatima, Spröer Cathrin, Lemos Paulo Costa, Besson Stephane, El Asli Abdel-Ghani, Bennisse Rhizlane, Labat Marc, Reis Maria, Qatibi Abdel-Illah
Anaerobic Microbiology Team (E02B26), Sciences and Techniques Faculty, Cadi Ayyad University, PO Box 549, 40 000 Marrakech, Morocco.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):936-42. doi: 10.1099/ijs.0.003822-0.
A novel mesophilic sulfate-reducing bacterium, EMSSDQ(4)(T), was isolated from olive mill wastewater in the semi-arid region of Morocco (Marrakech). Cells were Gram-negative, catalase-positive, straight rods that were non-motile and non-spore-forming and contained cytochrome c(3) and desulfoviridin. The DNA G+C content was 65.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was a member of the genus Desulfovibrio with Desulfovibrio carbinoliphilus D41(T), Desulfovibrio alcoholivorans SPSN(T), Desulfovibrio fructosivorans JJ(T) and Desulfovibrio carbinolicus EDK82(T) as the most closely related strains with validly published names. In addition to the classical substrates used by Desulfovibrio species, the isolate oxidized 1,4-tyrosol, one of the most abundant phenolic compounds occurring in olive mill wastewater, to 4-hydroxyphenylacetate without ring cleavage. D. alcoholivorans SPSN(T) was also found to carry out this reaction. Under air, strain EMSSDQ(4)(T) exhibited limited growth on lactate and yeast extract in the absence of sulfate. On the basis of genotypic and phenotypic characteristics, it is proposed that the isolate represents a novel species, Desulfovibrio marrakechensis sp. nov. The type strain is EMSSDQ(4)(T) (=DSM 19337(T) =ATCC BAA-1562(T)).
从摩洛哥半干旱地区（马拉喀什）的橄榄榨油厂废水中分离出一种新型嗜温硫酸盐还原菌，EMSSDQ(4)(T)。细胞为革兰氏阴性、过氧化氢酶阳性、直杆状，不运动、不产芽孢，含有细胞色素c(3)和脱硫绿素。DNA的G+C含量为65.1 mol%。基于16S rRNA基因序列的系统发育分析表明，该分离株是脱硫弧菌属的成员，与已有效发表名称的最密切相关菌株为嗜甲醇脱硫弧菌D41(T)、嗜乙醇脱硫弧菌SPSN(T)、嗜果糖脱硫弧菌JJ(T)和乙醇脱硫弧菌EDK82(T)。除了脱硫弧菌属使用的经典底物外，该分离株还将橄榄榨油厂废水中最丰富的酚类化合物之一1,4-酪醇氧化为4-羟基苯乙酸，且无环裂解。还发现嗜乙醇脱硫弧菌SPSN(T)也能进行此反应。在空气中，菌株EMSSDQ(4)(T)在无硫酸盐的情况下，在乳酸盐和酵母提取物上生长有限。基于基因型和表型特征，建议将该分离株代表一个新物种，马拉喀什脱硫弧菌新种。模式菌株为EMSSDQ(4)(T)（=DSM 19337(T)=ATCC BAA-1562(T)）。
