Chen Li-Hua, Duan Qun-Jun, Cai Mei-Ting, Wu Yi-Dong, Shang Shi-Qiang
Neonatal Department, Children's Hospital, Medical School, Zhejiang University, Hangzhou, China.
Clin Pediatr (Phila). 2009 Jul;48(6):641-7. doi: 10.1177/0009922809333972. Epub 2009 Apr 30.
A method for the detection of bacterial pathogens in sepsis and bacterial meningitis with 16S rRNA gene- based real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) is developed. A total of 190 blood specimens and 5 cerebrospinal fluid specimens from neonates with suspected sepsis or bacterial meningitis were evaluated with 16S rRNA gene-based real-time FQ-PCR assay. The positive rate of the real-time FQ-PCR assay was significantly higher (25/195, 12.82%) than that of bacterial culture (15/195, 7.69%; P = .002). When bacterial culture was used as a control, the sensitivity of the real-time FQ-PCR was 100%, the specificity was 94.4%, and Youden's index was 0.944. This study suggests that 16S rRNA gene-based real-time FQ-PCR assay is an important and accurate method in the detection of bacterial pathogens of sepsis and bacterial meningitis and should have a promising usage in the diagnosis of sepsis and bacterial meningitis.
开发了一种基于16S rRNA基因的实时荧光定量聚合酶链反应（FQ-PCR）检测败血症和细菌性脑膜炎中细菌病原体的方法。对190份来自疑似败血症或细菌性脑膜炎新生儿的血液标本和5份脑脊液标本进行了基于16S rRNA基因的实时FQ-PCR检测。实时FQ-PCR检测的阳性率（25/195，12.82%）显著高于细菌培养的阳性率（15/195，7.69%；P = 0.002）。以细菌培养为对照时，实时FQ-PCR的敏感性为100%，特异性为94.4%，约登指数为0.944。本研究表明，基于16S rRNA基因的实时FQ-PCR检测是检测败血症和细菌性脑膜炎细菌病原体的一种重要且准确的方法，在败血症和细菌性脑膜炎的诊断中应具有广阔的应用前景。
