Faculty of Chemical Engineering and Technology, University of Zagreb, Savska c. 16, 10000 Zagreb, Croatia.
Bioprocess Biosyst Eng. 2010 Mar;33(3):299-307. doi: 10.1007/s00449-009-0324-y. Epub 2009 May 1.
A commercial enzyme Dextrozyme was tested as catalyst for maltose hydrolysis at two different temperatures: 40 and 65 degrees C at pH 5.5. Its operational stability was studied in different reactor types: batch, repetitive batch, fed-batch and continuously operated enzyme membrane reactor. Dextrozyme was more active at 65 degrees C, but operational stability decay was observed during the prolonged use in the reactor at this temperature. The reactor efficiencies were compared according to the volumetric productivity, biocatalyst productivity and enzyme consumption. The best reactor type according to the volumetric productivity for maltose hydrolysis is batch and the best reactor type according to the biocatalyst productivity and enzyme consumption is continuously operated enzyme membrane reactor. The mathematical model developed for the maltose hydrolysis in the different reactors was validated by the experiments at both temperatures. The Michaelis-Menten kinetics describing maltose hydrolysis was used.
商业酶 Dextrozyme 在两种不同温度（40 和 65°C，pH5.5）下作为麦芽糖水解的催化剂进行了测试。研究了其在不同反应器类型（分批、重复分批、补料分批和连续操作酶膜反应器）中的操作稳定性。Dextrozyme 在 65°C 时更活跃，但在该温度下长时间在反应器中使用时，观察到操作稳定性下降。根据比生产率、生物催化剂生产率和酶消耗，比较了反应器的效率。根据麦芽糖水解的比生产率，最佳的反应器类型是分批式，而根据生物催化剂生产率和酶消耗，最佳的反应器类型是连续操作酶膜反应器。通过在两种温度下的实验验证了在不同反应器中开发的麦芽糖水解数学模型。使用了描述麦芽糖水解的米氏动力学。
