Buchmann William, Boutorine Alexandre, Halby Ludovic, Tortajada Jeanine, De Pauw Edwin
Université d'Evry val d'Essonne, Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement, (CNRS UMR 8587/CEA), Bâtiment Maupertuis, Bd. François Mitterrand, 91025 Evry, France.
J Mass Spectrom. 2009 Aug;44(8):1171-81. doi: 10.1002/jms.1592.
A new method for the determination of the relative affinity of a ligand against various dsDNA sequences is presented by using electrospray ionization time-of-flight mass spectrometry (ESI-QTOF) mass spectrometry. The principle is described here through the complexation of double-stranded DNA by a polyamide ligand including twelve N-methylpyrrole rings. However this method could be applied to other ligands especially when dissociation constants (Kd) are in nanomolar range. This method does not require knowing the ligand concentration accurately. It allows determination of the relative affinity of a ligand against various dsDNA sequences for 1 : 1 complex stoichiometries in a quick manner without labeling.
本文介绍了一种利用电喷雾电离飞行时间质谱（ESI-QTOF）测定配体与各种双链DNA序列相对亲和力的新方法。这里通过一种包含十二个N-甲基吡咯环的聚酰胺配体与双链DNA的络合作用来描述其原理。然而，该方法可应用于其他配体，特别是当解离常数（Kd）处于纳摩尔范围内时。此方法不需要精确知道配体浓度。它能够快速测定配体与各种双链DNA序列在1:1络合物化学计量比下的相对亲和力，且无需标记。
