Cotter John J, O'Gara James P, Casey Eoin
UCD School of Chemical and Bioprocess Engineering, Engineering and Materials Science Centre, Centre for Synthesis and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
Biotechnol Bioeng. 2009 Aug 1;103(5):1042-7. doi: 10.1002/bit.22335.
Biofilm-related research using 96-well microtiter plates involves static incubation of plates indiscriminate of environmental conditions, making oxygen availability an important variable which has not been considered to date. By directly measuring dissolved oxygen concentration over time we report here that dissolved oxygen is rapidly consumed in Staphylococcus epidermidis biofilm cultures grown in 96-well plates irrespective of the oxygen concentration in the gaseous environment in which the plates are incubated. These data indicate that depletion of dissolved oxygen during growth of bacterial biofilm cultures in 96-well plates may significantly influence biofilm production. Furthermore higher inoculum cell concentrations are associated with more rapid consumption of dissolved oxygen and higher levels of S. epidermidis biofilm production. Our data reveal that oxygen depletion during bacterial growth in 96-well plates may significantly influence biofilm production and should be considered in the interpretation of experimental data using this biofilm model.
使用96孔微量滴定板进行的生物膜相关研究涉及在不考虑环境条件的情况下对平板进行静态培养，使得氧的可利用性成为一个至今尚未被考虑的重要变量。通过直接测量随时间变化的溶解氧浓度，我们在此报告，在96孔板中生长的表皮葡萄球菌生物膜培养物中，溶解氧会迅速消耗，而与培养平板所处气态环境中的氧浓度无关。这些数据表明，在96孔板中细菌生物膜培养物生长过程中溶解氧的消耗可能会显著影响生物膜的形成。此外，较高的接种细胞浓度与溶解氧的更快消耗以及更高水平的表皮葡萄球菌生物膜形成相关。我们的数据表明，在96孔板中细菌生长过程中的氧消耗可能会显著影响生物膜的形成，并且在使用该生物膜模型解释实验数据时应予以考虑。
