[Effect of rat recombinant leptin on expression of SOCS-3 in mature adipocytes].

OBJECTIVE

To study the effect of rat recombinant leptin on expressions of suppressor of cytokine signaling 3 (SOCS-3) mRNA and protein in mature adipocytes .

METHODS

Mature adipocytes were separated from epididymal adipose tissues of male SD rats, and the expressions of long-form leptin receptor mRNA were detected. The separated adipocytes were then treated with rat recombinant leptin at the doses of 0.5-500 nmol/L for 0.5 to 6h. The expressions of SOCS-3 mRNA were measured with RT-PCR and proteins were measured with immuniprecipitation combined with Western blot analysis.

RESULTS

Low-level expressions of long-form leptin receptor mRNA could be measured in mature adipocytes. The adipocytes expressed small amounts of SOCS-3 mRNA and protein witbout leptin treatment, and no significant changes were observed at 0.5 Snmol/L of leptin. However, tbe expressions of SOCS-3 mRNA and protein increased significantly at the doses of 5-500 nmol/L of leptin in the consistent with treatment time.

CONCLUSION

Leptin could have direct effects on adipocytes. High leptin concentration could induce the expressions of SOCS-3 mRNA and protein in mature adipocytes.