Gao Mei-Juan, Liu Ming, Li Bo, Li Ming-Long, Bian Li-Xiang, Yu Gui-Na
Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Yao Xue Xue Bao. 2009 Feb;44(2):126-33.
The aim of this study is to study the effect of calcium dobesilate on streptozotocin (STZ)-induced early diabetic nephrophathy (DN) in rats. All male Wistar rats were randomly divided into six groups: normal group; DN blank group; calcium dobesilate 75, 150, and 300 mg x kg(-1) groups and perindopril 0.4 mg x kg(-1) group. Blood glucose and the 24 h urinary albumin were measured dynamically during the experiment, after 8 weeks administration, the level of glycosylated hemoglobin (HbA1c) was determined, the expressions of plasminogen activator inhibitor-1 (PAI-1) and matrix metalloprotein-9 (MMP-9) in cortex of kidney were examined with immunohistochemical staining. The endothelin (ET) in plasma and kidney cortex was measured with radioimmunoassay, renal pathomorphism was observed with light and electron microscopes. Calcium dobesilate could decrease the 24 h urinary albumin and ET in plasma and kidney cortex, down-regulate the expression of PAI-1, and up-regulate MMP-9 in kidney. These findings suggested that calcium dobesilate could protect blood vessel endothelium, inhibit kidney fibrous degeneration, ameliorate renal pathological damage, and protect kidney function in many ways.
本研究旨在探讨羟苯磺酸钙对链脲佐菌素（STZ）诱导的大鼠早期糖尿病肾病（DN）的影响。将所有雄性Wistar大鼠随机分为6组：正常组；DN空白组；羟苯磺酸钙75、150和300 mg·kg⁻¹组以及培哚普利0.4 mg·kg⁻¹组。实验期间动态检测血糖和24 h尿白蛋白，给药8周后测定糖化血红蛋白（HbA1c）水平，采用免疫组织化学染色法检测肾皮质中纤溶酶原激活物抑制剂-1（PAI-1）和基质金属蛋白酶-9（MMP-9）的表达。采用放射免疫法测定血浆和肾皮质中的内皮素（ET），用光镜和电镜观察肾脏病理形态。羟苯磺酸钙可降低24 h尿白蛋白及血浆和肾皮质中的ET水平，下调PAI-1表达，上调肾脏中MMP-9表达。这些结果表明，羟苯磺酸钙可通过多种方式保护血管内皮，抑制肾脏纤维变性，改善肾脏病理损伤，保护肾脏功能。
