Wang Libing, Zhu Yingyue, Jiang Yuan, Qiao Ruirui, Zhu Shuifang, Chen Wei, Xu Chuanlai
Hunan Import and Export Inspection and Quarantine Bureau, Changsha, HuNan, 410000, PRC.
J Phys Chem B. 2009 May 28;113(21):7637-41. doi: 10.1021/jp902404y.
The effects of quantum dots (QDs) on the elimination of nonspecific amplification of the polymerase chain reaction (PCR) were investigated. It was found that QDs could increase the specificity of the PCR at different annealing temperatures and with DNA templates of different lengths. The effects of QDs on the efficiency of the PCR were also studied, and the results showed that there was no enhancement. The mechanisms underlying these effects are discussed. This method could be used to modify the amplification results of the conventional PCR. Furthermore, this technology could make the PCR more widely applicable, especially in the multi-PCR reaction system with different annealing temperatures. This is of great significance for gene diagnosis.
研究了量子点（QDs）对消除聚合酶链反应（PCR）非特异性扩增的影响。发现量子点在不同退火温度下以及使用不同长度的DNA模板时均可提高PCR的特异性。还研究了量子点对PCR效率的影响，结果表明没有增强作用。讨论了这些效应背后的机制。该方法可用于改变传统PCR的扩增结果。此外，该技术可使PCR更广泛地适用，尤其是在具有不同退火温度的多重PCR反应体系中。这对基因诊断具有重要意义。
