Piñeiro Luis, Montes Milagrosa, Gil-Setas Alberto, Camino Xabier, Echeverria María Julia, Cilla Gustavo
Servicio de Microbiología, Hospital Donostia, San Sebastián, Gipuzkoa, España.
Enferm Infecc Microbiol Clin. 2009 Oct;27(8):462-4. doi: 10.1016/j.eimc.2008.12.012. Epub 2009 May 1.
Circulating Chlamydia trachomatis genotypes that cause infection in our geographic area were studied with the aim of detecting possible epidemiological peculiarities.
A total of 177 strains obtained between 2006 and 2008 were genotyped using a PCR with primers targeting the ompA gene, and later sequenced.
The most frequent genotypes were: E (45.3%), D (15.3%), G (10.2%) and F (9.6%). Other genotypes found were: B, H, I, J, K and LGV II.
The molecular assay used had a high yield (89%).
为了检测可能存在的流行病学特征,我们对在我们所在地理区域引起感染的沙眼衣原体循环基因型进行了研究。
使用针对ompA基因的引物通过聚合酶链反应(PCR)对2006年至2008年间获得的总共177株菌株进行基因分型,随后进行测序。
最常见的基因型为:E(45.3%)、D(15.3%)、G(L0.2%)和F(9.6%)。发现的其他基因型有:B、H、I、J、K和LGV II。
所使用的分子检测方法具有较高的检出率(89%)。