Kim Bieong-Kil, Doh Kyung-Oh, Nam Joo Hyeung, Kang Hyungu, Park Jong-Gu, Moon Ik-Jae, Seu Young-Bae
School of Life Sciences and Biotechnology, Kyungpook National University, Sankyukdong, Bookgu, Daegu, Republic of Korea.
Bioorg Med Chem Lett. 2009 Jun 1;19(11):2986-9. doi: 10.1016/j.bmcl.2009.04.036. Epub 2009 Apr 18.
The new cholesterol-based cationic lipids B, C, and D with an ether linked spacer were synthesized by using aminopropyl chain extension with acrylonitrile. The cholesterol-based cationic lipid A with carbamoyl linkage were also synthesized in order to compare the difference in transfection efficiency of the two linkage types. To this end, GFP expression of these cationic lipids was confirmed respectively.
通过丙烯腈进行氨丙基链延长反应，合成了带有醚键连接间隔基的新型胆固醇基阳离子脂质B、C和D。还合成了具有氨基甲酰基连接的胆固醇基阳离子脂质A，以比较两种连接类型在转染效率上的差异。为此，分别确认了这些阳离子脂质的绿色荧光蛋白（GFP）表达情况。
