Wei Shu-mei, Xie Chuan-gao, Abe Yasuhito, Cai Jian-ting
Department of Pathology, Second Affiliated Hospital of Zhejiang University, School of Medicine, Jiefang Road 88[#], Hangzhou, Zhejiang Province 310009, China.
Biochem Biophys Res Commun. 2009 Jul 3;384(3):352-6. doi: 10.1016/j.bbrc.2009.04.125. Epub 2009 May 3.
ADP-ribosylation factor (ARF) like 7 (ARL7, also named ARL4C) is a member of ARL family and recent studies showed that it is involved in the AI-dependent cholesterol secretion process. Yet its biological function remains largely unknown. Using a MALDI-TOF/MS analysis, we identified alpha-tubulin interacted with ARL7. The interaction was confirmed by GST pull-down assay and co-immunoprecipitation in renal carcinoma cell 786-O in which we found the endogenous ARL7 is expressed. This is the second ARL member found interacting with tubulin after ARL8. In addition, ARL7Q72L, a GTP-binding form, promoted the transferrin transport from early endosome to recycling endosome significantly. The above data suggested that ARL7 might modulate the intracellular vesicular transport via interaction with microtubules.
ADP-核糖基化因子样蛋白7（ARL7，也称为ARL4C）是ARL家族的成员，最近的研究表明它参与了依赖AI的胆固醇分泌过程。然而，其生物学功能在很大程度上仍然未知。通过基质辅助激光解吸电离飞行时间质谱分析，我们鉴定出α-微管蛋白与ARL7相互作用。在表达内源性ARL7的肾癌细胞786-O中，通过谷胱甘肽S-转移酶下拉实验和免疫共沉淀证实了这种相互作用。这是继ARL8之后发现的第二个与微管蛋白相互作用的ARL家族成员。此外，GTP结合形式的ARL7Q72L显著促进了转铁蛋白从早期内体向再循环内体的转运。上述数据表明，ARL7可能通过与微管相互作用来调节细胞内囊泡运输。
