ADP核糖基化因子样7（ARL7）与α微管蛋白相互作用并调节细胞内囊泡运输。

ADP-ribosylation factor like 7 (ARL7) interacts with alpha-tubulin and modulates intracellular vesicular transport.

作者信息

Wei Shu-mei, Xie Chuan-gao, Abe Yasuhito, Cai Jian-ting

机构信息

Department of Pathology, Second Affiliated Hospital of Zhejiang University, School of Medicine, Jiefang Road 88[#], Hangzhou, Zhejiang Province 310009, China.

出版信息

Biochem Biophys Res Commun. 2009 Jul 3;384(3):352-6. doi: 10.1016/j.bbrc.2009.04.125. Epub 2009 May 3.

DOI:10.1016/j.bbrc.2009.04.125

PMID:19409876

Abstract

ADP-ribosylation factor (ARF) like 7 (ARL7, also named ARL4C) is a member of ARL family and recent studies showed that it is involved in the AI-dependent cholesterol secretion process. Yet its biological function remains largely unknown. Using a MALDI-TOF/MS analysis, we identified alpha-tubulin interacted with ARL7. The interaction was confirmed by GST pull-down assay and co-immunoprecipitation in renal carcinoma cell 786-O in which we found the endogenous ARL7 is expressed. This is the second ARL member found interacting with tubulin after ARL8. In addition, ARL7Q72L, a GTP-binding form, promoted the transferrin transport from early endosome to recycling endosome significantly. The above data suggested that ARL7 might modulate the intracellular vesicular transport via interaction with microtubules.

摘要

ADP-核糖基化因子样蛋白7（ARL7，也称为ARL4C）是ARL家族的成员，最近的研究表明它参与了依赖AI的胆固醇分泌过程。然而，其生物学功能在很大程度上仍然未知。通过基质辅助激光解吸电离飞行时间质谱分析，我们鉴定出α-微管蛋白与ARL7相互作用。在表达内源性ARL7的肾癌细胞786-O中，通过谷胱甘肽S-转移酶下拉实验和免疫共沉淀证实了这种相互作用。这是继ARL8之后发现的第二个与微管蛋白相互作用的ARL家族成员。此外，GTP结合形式的ARL7Q72L显著促进了转铁蛋白从早期内体向再循环内体的转运。上述数据表明，ARL7可能通过与微管相互作用来调节细胞内囊泡运输。