Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
Arch Environ Contam Toxicol. 2010 Jan;58(1):62-70. doi: 10.1007/s00244-009-9329-4. Epub 2009 May 6.
This study was conducted to find an appropriate approach for the assessment of bioavailability of DDTs in soil to both earthworm and vegetables. Four chemical approaches--Soxhlet extraction with n-hexane, n-butanol agitation extraction, water agitation extraction, and matrix solid-phase microextraction (matrix-SPME)--were used to assess the relationships between the extractability of 1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene (p,p'-DDE), 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethane (o,p'-DDT), 1,1-dichloro-2,2-bis(p-chlorophenyl) ethane (p,p'-DDD), and 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane (p,p'-DDT) in soil and their amounts uptaken by the earthworm (Eisenia foetida), Chinese cabbage (Brassica campestris L. spp.), and cole (Brassica napus L.). These results indicated that the extractability and bioavailability of DDTs in soil decreased with time of aging. Correlation analysis showed that n-butanol extraction or 12-h matrix-SPME could be used to assess the bioavailability of DDTs to the earthworm, and Soxhlet extraction, n-butanol extraction, or 12-h matrix-SPME could be used to predict the bioavailability of DDTs to both Chinese cabbage and cole. As a solventless, time-efficient, and negligible-depletion technique, it could be concluded that matrix-SPME is a better approach to predict the bioavailability of DDTs to both the earthworm and vegetables, compared with Soxhlet extraction, n-butanol extraction, and water extraction.
本研究旨在寻找一种合适的方法来评估滴滴涕（DDTs）在土壤中对蚯蚓和蔬菜的生物可利用性。采用索氏提取（正己烷）、正丁醇搅拌提取、水搅拌提取和基质固相微萃取（matrix-SPME）四种化学方法来评估 1,1-二氯-2,2-双（对氯苯基）乙烯（p,p'-DDE）、1,1,1-三氯-2-（对氯苯基）-2-（邻氯苯基）乙烷（o,p'-DDT）、1,1-二氯-2,2-双（对氯苯基）乙烷（p,p'-DDD）和 1,1,1-三氯-2,2-双（对氯苯基）乙烷（p,p'-DDT）在土壤中的可提取性与其被蚯蚓（赤子爱胜蚓）、白菜（ Brassica campestris L. spp.）和油菜（ Brassica napus L.）吸收量之间的关系。结果表明，土壤中 DDTs 的可提取性和生物可利用性随老化时间的延长而降低。相关分析表明，正丁醇提取或 12 小时 matrix-SPME 可用于评估 DDTs 对蚯蚓的生物可利用性，而索氏提取、正丁醇提取或 12 小时 matrix-SPME 可用于预测 DDTs 对白菜和油菜的生物可利用性。作为一种无溶剂、高效、微量损耗的技术，可以得出结论，与索氏提取、正丁醇提取和水提取相比，matrix-SPME 是一种更好的方法来预测 DDTs 对蚯蚓和蔬菜的生物可利用性。
