Conway M D, Davison-Fairburn B, Martin L N, Insler M S, Murphey-Corb M
LSU Eye Center, New Orleans, LA.
J Med Primatol. 1991 Jun;20(4):152-5.
We tested the ability of SIV to cause local and systemic infection in three rhesus monkeys after topical instillation of cell-free virus into the conjunctival cul-de-sac. Conjunctivitis or other signs of infection were monitored after inoculation. Conjunctiva were swabbed for virus culture and biopsied for PCR. Changes in lymphocyte subsets, seroconversion, antigenemia, and virus isolation from PBL were assessed systemically postinoculation. Viral DNA was detected in conjunctival biopsy by PCR in one of three animals that later developed systemic infection. The other two animals remained uninfected. These data demonstrate that the conjunctiva is a route by which SIV (and perhaps HIV) may cause systemic infection.
我们将无细胞病毒局部滴入三只恒河猴的结膜穹窿，测试了猴免疫缺陷病毒（SIV）引发局部和全身感染的能力。接种后监测结膜炎或其他感染迹象。采集结膜拭子进行病毒培养，并取活检组织进行聚合酶链反应（PCR）检测。接种后系统评估淋巴细胞亚群、血清转化、抗原血症以及从外周血淋巴细胞（PBL）中分离病毒的变化。在三只后来发生全身感染的动物中，有一只通过PCR在结膜活检组织中检测到病毒DNA。另外两只动物未被感染。这些数据表明，结膜是SIV（或许还有HIV）可能引发全身感染的一条途径。
