Yoo Chan-Jong, Yoo Young-Mi, Kim Yong-Jung
Department of Neurosurgery, Gachon Medical College, Gil Medical Center, Incheon, South Korea.
Neurosci Lett. 2009 Jul 3;457(3):151-4. doi: 10.1016/j.neulet.2009.03.056. Epub 2009 Mar 21.
After spinal cord injury (SCI), a loss of myelinating oligodendrocytes and neurons occurs. The functional recovery of injured spinal cords is the principal objective of SCI repair. Cell transplantation may prove beneficial to help replace lost myelin and spinal cord circuitry. In this study, we demonstrated that neural precursor cells (hNPCs) from human fetal spinal cord express three types of proteoglycan proteins-chondroitin sulfate, keratan sulfate, and cartilage proteoglycan (an extracellular matrix detected in normal spinal cord), and non-proteoglycan matrix collagen. Both proteoglycan and collagen evidenced profound immunoreactivity in double-stained cell clusters. In addition, whether or not hNPCs were capable of differentiating into a variety of cells, including GABAergic and cholinergic neurons, were assessed. The differentiated cells of eight passages grown on a monolayer expressed the human nuclear protein (HNu), the progenitor marker nestin, GAD, ChAT, TJU, and MAP-2. These results indicate that hNPCs may prove to be candidate cells for therapeutic SCI strategies.
脊髓损伤（SCI）后，会出现有髓少突胶质细胞和神经元的丧失。损伤脊髓的功能恢复是SCI修复的主要目标。细胞移植可能有助于替代丢失的髓鞘和脊髓神经回路。在本研究中，我们证明来自人胎儿脊髓的神经前体细胞（hNPCs）表达三种蛋白聚糖蛋白——硫酸软骨素、硫酸角质素和软骨蛋白聚糖（在正常脊髓中检测到的一种细胞外基质），以及非蛋白聚糖基质胶原蛋白。蛋白聚糖和胶原蛋白在双染细胞簇中均表现出强烈的免疫反应性。此外，还评估了hNPCs是否能够分化为包括GABA能和胆碱能神经元在内的多种细胞。在单层上生长的八代分化细胞表达人核蛋白（HNu）、祖细胞标志物巢蛋白、谷氨酸脱羧酶（GAD）、胆碱乙酰转移酶（ChAT）、天津大学标志物（TJU）和微管相关蛋白2（MAP-2）。这些结果表明，hNPCs可能是SCI治疗策略的候选细胞。
