Gwon Song-Hui, Hwang Jae-Kwan
Department of Biotechnology, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea.
J Ethnopharmacol. 2009 Jun 22;123(2):315-24. doi: 10.1016/j.jep.2009.02.047. Epub 2009 Mar 9.
Kaempferia pandurata Roxb., an edible tropical medicinal plant (Zingiberaceae), has been traditionally used to treat dental caries. However, its efficacy on inhibition of matrix metalloproteinase (MMP)-9 for preventing periodontal inflammation has been rarely studied to date.
This present study was focused to investigate the effects of the ethanolic extract of Kaempferia pandurata Roxb. on inhibition of Porphyromonas gingivalis supernatant-induced MMP-9 expression through activation of mitogen-activated protein kinase (MAPK) signaling pathways in human oral epidermoid KB cells.
MMP-9 expression and its signaling pathways in KB cells induced by Porphyromonas gingivalis supernatant were determined by gelatin zymogram, Western blotting and reporter gene assays.
Kaempferia pandurata significantly decreased MMP-9 expression at both protein and mRNA levels in a dose-dependent manner. Kaempferia pandurata interfered Porphyromonas gingivalis supernatant-induced MMP-9 expression in KB cells by downregulating MAPK phosphorylation (extracellular signal-related kinase 1/2, p38 kinase and c-Jun N-terminal kinase), inhibiting transcriptional expression (Elk1, c-Jun and c-Fos), and blocking AP-1 and NF-kappaB activities.
Kaempferia pandurata could be employed as a candidate for MMP-9 inhibitor with therapeutic potential for treatment of periodontal inflammation.
莪术（姜科一种可食用的热带药用植物）传统上用于治疗龋齿。然而，其对抑制基质金属蛋白酶（MMP）-9以预防牙周炎的功效迄今鲜有研究。
本研究旨在探讨莪术乙醇提取物通过激活人口腔表皮样KB细胞中的丝裂原活化蛋白激酶（MAPK）信号通路，对牙龈卟啉单胞菌上清液诱导的MMP-9表达的抑制作用。
采用明胶酶谱法、蛋白质印迹法和报告基因分析法，测定牙龈卟啉单胞菌上清液诱导的KB细胞中MMP-9的表达及其信号通路。
莪术能以剂量依赖性方式显著降低MMP-9在蛋白质和mRNA水平的表达。莪术通过下调MAPK磷酸化（细胞外信号调节激酶1/2、p38激酶和c-Jun氨基末端激酶）、抑制转录表达（Elk1、c-Jun和c-Fos）以及阻断AP-１和核因子κB活性，干扰牙龈卟啉单胞菌上清液诱导的KB细胞中MMP-9的表达。
莪术可作为MMP-9抑制剂的候选药物，具有治疗牙周炎的潜在治疗价值。
