Kawaguchi H, Noda H
J Biochem. 1977 May;81(5):1307-17.
Thermal denaturation of cytochromes c of horse, cow, and Candida krusei in aqueous guanidine hydrochloride in the neutral pH region was studied by means of absorption and optical rotation measurements. The values of standard free energy change upon denaturation were estimated over the temperature range from 3 to 51 degrees C. Large differences in the heat capacity of the native and denatured states amounting to several kcal/mol-deg were obtained for all three kinds of cytochromes c. These lead to a change in the sign of both the enthalpy and entropy change of denaturation, with maximum stability of the native state at 12 degrees C for horse and bovine cytochromes c and at 9 degrees C for Candida krusei.
通过吸收和旋光测量研究了马、牛和克鲁斯假丝酵母的细胞色素c在中性pH值区域的盐酸胍水溶液中的热变性。在3至51摄氏度的温度范围内估计了变性时的标准自由能变化值。对于所有三种细胞色素c，天然态和变性态的热容存在很大差异，达数千卡/摩尔-摄氏度。这些导致变性焓和熵变化的符号发生变化，马和牛细胞色素c的天然态在12摄氏度时稳定性最高，克鲁斯假丝酵母的在9摄氏度时稳定性最高。
