皮炎外瓶霉（枝孢瓶霉）V类几丁质合酶的免疫亲和纯化
Immunoaffinity purification of the class V chitin synthase of Wangiella (Exophiala) dermatitidis.
作者信息
Abramczyk Dariusz, Szaniszlo Paul J
机构信息
Section of Molecular Genetics and Microbiology, School of Biological Science and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA.
出版信息
Prep Biochem Biotechnol. 2009;39(3):277-88. doi: 10.1080/10826060902953244.
Abstract
The class V chitin synthase is unique because it has a myosin motor-like domain fused to its catalytic domain. The biochemical properties of this enzyme and its function remain undefined beyond the knowledge that it is the only single chitin synthase required for sustained cell growth at elevated temperatures and, consequently, virulence. This report describes our successful efforts to isolate and purify an active and soluble form of the enzyme from the cell membranes of Wangiella by using a specific polyclonal antibody. To our knowledge, this is the first purification of a single chitin synthase of a filamentous fungus.
摘要
V类几丁质合酶很独特，因为它的催化结构域融合了一个类似肌球蛋白马达的结构域。除了知道它是高温下持续细胞生长以及毒力所需的唯一一种单一几丁质合酶外，这种酶的生化特性及其功能仍不明确。本报告描述了我们通过使用特异性多克隆抗体，成功地从弯孢霉菌细胞膜中分离并纯化出该酶的活性可溶形式。据我们所知，这是丝状真菌单一几丁质合酶的首次纯化。
相似文献
1
Immunoaffinity purification of the class V chitin synthase of Wangiella (Exophiala) dermatitidis.
Prep Biochem Biotechnol. 2009;39(3):277-88. doi: 10.1080/10826060902953244.
2
Cytolocalization of the class V chitin synthase in the yeast, hyphal and sclerotic morphotypes of Wangiella (Exophiala) dermatitidis.
Fungal Genet Biol. 2009 Jan;46(1):28-41. doi: 10.1016/j.fgb.2008.10.004. Epub 2008 Oct 21.
3
Transcription and expression analyses of WdCHS5, which encodes a class V chitin synthase with a myosin motor-like domain in Wangiella (Exophiala) dermatitidis.
FEMS Microbiol Lett. 2007 Nov;276(1):99-105. doi: 10.1111/j.1574-6968.2007.00920.x.
4
WdCHS3, a gene that encodes a class III chitin synthase in Wangiella (Exophiala) dermatitidis, is expressed differentially under stress conditions.
J Bacteriol. 2000 Feb;182(4):874-81. doi: 10.1128/JB.182.4.874-881.2000.
5
Wangiella (Exophiala) dermatitidis WdChs5p, a class V chitin synthase, is essential for sustained cell growth at temperature of infection.
Eukaryot Cell. 2004 Feb;3(1):40-51. doi: 10.1128/EC.3.1.40-51.2004.
6
WdChs4p, a homolog of chitin synthase 3 in Saccharomyces cerevisiae, alone cannot support growth of Wangiella (Exophiala) dermatitidis at the temperature of infection.
Infect Immun. 1999 Dec;67(12):6619-30. doi: 10.1128/IAI.67.12.6619-6630.1999.
7
Characterization of WdChs3p, a class III chitin synthase, of Wangiella (Exophiala) dermatitidis, overexpressed in Saccharomyces cerevisiae.
Med Mycol. 2002 Jun;40(3):283-9. doi: 10.1080/mmy.40.3.283.289.
8
WdChs2p, a class I chitin synthase, together with WdChs3p (class III) contributes to virulence in Wangiella (Exophiala) dermatitidis.
Infect Immun. 2001 Dec;69(12):7517-26. doi: 10.1128/IAI.69.12.7517-7526.2001.
9
Compensatory expression of five chitin synthase genes, a response to stress stimuli, in Wangiella (Exophiala) dermatitidis, a melanized fungal pathogen of humans.
Microbiology (Reading). 2002 Sep;148(Pt 9):2811-2817. doi: 10.1099/00221287-148-9-2811.
10
WdChs1p, a class II chitin synthase, is more responsible than WdChs2p (Class I) for normal yeast reproductive growth in the polymorphic, pathogenic fungus Wangiella (Exophiala) dermatitidis.
Arch Microbiol. 2006 May;185(4):316-29. doi: 10.1007/s00203-006-0101-0. Epub 2006 Mar 17.
引用本文的文献
1
: Key issues of an opportunistic fungal pathogen.
Virulence. 2019 Dec;10(1):984-998. doi: 10.1080/21505594.2019.1596504. Epub 2019 Apr 3.
2
Black yeasts and their filamentous relatives: principles of pathogenesis and host defense.
Clin Microbiol Rev. 2014 Jul;27(3):527-42. doi: 10.1128/CMR.00093-13.
3
Expression in E. coli and characterization of the catalytic domain of Botrytis cinerea chitin synthase.
BMC Res Notes. 2010 Nov 11;3:299. doi: 10.1186/1756-0500-3-299.
本文引用的文献
1
Transcription and expression analyses of WdCHS5, which encodes a class V chitin synthase with a myosin motor-like domain in Wangiella (Exophiala) dermatitidis.
FEMS Microbiol Lett. 2007 Nov;276(1):99-105. doi: 10.1111/j.1574-6968.2007.00920.x.
2
Chitosomes: past, present and future.
FEMS Yeast Res. 2006 Nov;6(7):957-65. doi: 10.1111/j.1567-1364.2006.00158.x. Epub 2006 Sep 19.
3
The structure and synthesis of the fungal cell wall.
Bioessays. 2006 Aug;28(8):799-808. doi: 10.1002/bies.20441.
4
Wangiella (Exophiala) dermatitidis WdChs5p, a class V chitin synthase, is essential for sustained cell growth at temperature of infection.
Eukaryot Cell. 2004 Feb;3(1):40-51. doi: 10.1128/EC.3.1.40-51.2004.
5
Evolution and phylogenetic relationships of chitin synthases from yeasts and fungi.
FEMS Yeast Res. 2002 Jan;1(4):247-56. doi: 10.1111/j.1567-1364.2002.tb00042.x.
6
Molecular genetic studies of the model dematiaceous pathogen Wangiella dermatitidis.
Int J Med Microbiol. 2002 Oct;292(5-6):381-90. doi: 10.1078/1438-4221-00221.
7
csmA, a gene encoding a class V chitin synthase with a myosin motor-like domain of Aspergillus nidulans, is translated as a single polypeptide and regulated in response to osmotic conditions.
Biochem Biophys Res Commun. 2002 Oct 18;298(1):103-9. doi: 10.1016/s0006-291x(02)02418-x.
8
Dynamics of cell wall structure in Saccharomyces cerevisiae.
FEMS Microbiol Rev. 2002 Aug;26(3):239-56. doi: 10.1111/j.1574-6976.2002.tb00613.x.
9
Chitin synthesis in human pathogenic fungi.
Med Mycol. 2001;39 Suppl 1:41-53.
10
WdChs2p, a class I chitin synthase, together with WdChs3p (class III) contributes to virulence in Wangiella (Exophiala) dermatitidis.
Infect Immun. 2001 Dec;69(12):7517-26. doi: 10.1128/IAI.69.12.7517-7526.2001.
Zotero 插件