大肠杆菌中核糖体的合成：I. 胞嘧啶-尿嘧啶掺入代谢库和RNA的过程
The Synthesis of Ribosomes in E. coli: I. The Incorporation of C-Uracil into the Metabolic Pool and RNA.
作者信息
McCarthy B J, Britten R J
出版信息
Biophys J. 1962 Jan;2(1):35-47. doi: 10.1016/s0006-3495(62)86839-8.
Abstract
C(14)-uracil is rapidly incorporated by E. coli at low concentrations. Approximately half the radioactivity passes directly into RNA with very little delay. The remaining half enters a large metabolic pool and later is incorporated into RNA. The total rate of uptake (growing cells) is not greater than the requirement for uracil and cytosine for RNA synthesis. The size of the metabolic pool is not influenced measurably by the external uracil concentration. No evidence is found for the existence of a fraction of RNA which is rapidly synthesized and degraded.
摘要
C¹⁴标记的尿嘧啶在低浓度时能被大肠杆菌迅速摄取。大约一半的放射性物质几乎没有延迟就直接进入RNA。其余一半进入一个大的代谢池，随后再被整合到RNA中。摄取的总速率（生长中的细胞）不超过RNA合成对尿嘧啶和胞嘧啶的需求。代谢池的大小不受外部尿嘧啶浓度的显著影响。未发现存在快速合成和降解的RNA部分的证据。
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