Hosek B, Bohácek J, Sikulová J
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Life Sci. 1991;49(19):1403-7. doi: 10.1016/0024-3205(91)90392-o.
Changes in ADA and PNP activities in the spleens and thymuses of mice were studied after a single administration of cyclophosphamide (CY, 200 mg/kg) and after whole-body gamma irradiation (5.5 Gy), applied alone or three days after CY application. In the first days after the treatment the enzyme activities were significantly depressed (p less than 0.01) with the exception of ADA in the spleen, where a high elevation (220-380%) in relation to controls was observed. During the regeneration period a pronounced rise of PNP activity in the spleen occurred mainly after a combined application of CY and irradiation (270%). In the thymus the regeneration was manifested by a mild increase of both ADA and PNP activities towards control values. The findings suggest that the expressive changes of ADA and PNP activities, participating in the purine salvage pathway, may, after a cytotoxic treatment, influence the nucleotide pool and DNA synthesis in lymphoid organs.
在单次给予环磷酰胺（CY，200mg/kg）后以及全身γ射线照射（5.5Gy）后，单独应用或在应用CY三天后应用，研究了小鼠脾脏和胸腺中ADA和PNP活性的变化。在治疗后的头几天，酶活性显著降低（p<0.01），脾脏中的ADA除外，在脾脏中观察到相对于对照组有高度升高（220-380%）。在再生期，脾脏中PNP活性显著升高主要发生在CY和照射联合应用后（270%）。在胸腺中，再生表现为ADA和PNP活性均轻度增加至对照值。这些发现表明，参与嘌呤补救途径的ADA和PNP活性的明显变化，在细胞毒性治疗后，可能会影响淋巴器官中的核苷酸池和DNA合成。
