Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis.
作者信息
McKenzie Fiona, Steven Victoria, Ingram Andrew, Graham Duncan
机构信息
Centre for Molecular Nanometrology, WestCHEM, Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
出版信息
Chem Commun (Camb). 2009 May 28(20):2872-4. doi: 10.1039/b823057a. Epub 2009 Mar 31.
DOI:10.1039/b823057a
PMID:19436894
Abstract
A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.
摘要
Zotero 插件