College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Anal Chim Acta. 2013 Jun 19;784:53-8. doi: 10.1016/j.aca.2013.04.038. Epub 2013 Apr 28.
Herein, fluorescent DNA-templated gold/silver nanoclusters (DNA-Au/Ag NCs) are presented as a novel probe for sensitive detection of deoxyribonuclease I (DNase I). The procedure is based on quenching fluorescence of DNA-Au/Ag NCs by DNase I digestion of the DNA (5'-CCCTTAATCCCC-3') template. This decrease in fluorescence intensity permitted sensitive detection of DNase I in a linear range of 0.013-60 μg mL(-1), with a detection limit of 3 ng mL(-1) at a signal-to-noise ratio of 3. Furthermore, the practicality of this probe for detection of DNase I in human serum and saliva samples was validated, demonstrating its advantages of simplicity, selectivity, sensitivity and low cost. Importantly, satisfactory agreement between results obtained by the fluorescent method described here and high performance liquid chromatography (HPLC) further confirmed the reliability and accuracy of this approach.
本文提出了一种新型荧光探针，用于灵敏检测脱氧核糖核酸酶 I（DNase I）。该探针基于荧光 DNA 模板金/银纳米簇（DNA-Au/Ag NCs）被 DNase I 消化的荧光猝灭原理。这种荧光强度的降低使得能够在 0.013-60μg mL(-1) 的线性范围内对 DNase I 进行灵敏检测，信噪比为 3 时检测限为 3ng mL(-1)。此外，该探针在人血清和唾液样本中检测 DNase I 的实用性也得到了验证，证明了该探针具有简单、选择性好、灵敏度高和成本低的优点。重要的是，这里描述的荧光方法与高效液相色谱（HPLC）得到的结果之间令人满意的一致性进一步证实了该方法的可靠性和准确性。
