Brennan Eoin P, Ehrich Mathias, Brazil Derek P, Crean John K, Murphy Madeline, Sadlier Denise M, Martin Finian, Godson Catherine, McKnight Amy Jayne, van den Boom Dirk, Maxwell Alexander P, Savage David A
Nephrology Research Group, Queen's University Belfast, Belfast, Northern, Ireland.
Epigenetics. 2009 Apr 1;4(3):159-64. doi: 10.4161/epi.4.3.8793. Epub 2009 Apr 18.
Previous reports have shown that DNA methylation profiles within primary human malignant tissues are altered when these cells are transformed into cancer cell lines. However, it is unclear if similar differences in DNA methylation profiles exist between DNA derived from peripheral blood leukocytes (PBLs) and corresponding Epstein-Barr Virus transformed lymphoblastoid cell lines (LCLs). To assess the utility of LCLs as a resource for methylation studies we have compared DNA methylation profiles in promoter and 5' regions of 318 genes in PBL and LCL sample pairs from patients with type 1 diabetes with or without nephropathy. We identified a total of 27 (approximately 8%) genes that revealed different DNA methylation profiles in PBL compared with LCL-derived DNA samples. In conclusion, although the profiles for most promoter regions were similar between PBL-LCL pairs, our results indicate that LCL-derived DNA may not be suitable for DNA methylation studies at least in diabetic nephropathy.
先前的报告显示，当原代人类恶性组织中的细胞转化为癌细胞系时，其DNA甲基化谱会发生改变。然而，尚不清楚来自外周血白细胞（PBL）的DNA与相应的爱泼斯坦-巴尔病毒转化的淋巴母细胞系（LCL）之间在DNA甲基化谱上是否存在类似差异。为了评估LCL作为甲基化研究资源的效用，我们比较了1型糖尿病伴或不伴肾病患者的PBL和LCL样本对中318个基因启动子和5'区域的DNA甲基化谱。我们共鉴定出27个（约8%）基因，这些基因在PBL中与LCL衍生的DNA样本相比显示出不同的DNA甲基化谱。总之，尽管大多数启动子区域在PBL-LCL对之间的谱相似，但我们的结果表明，至少在糖尿病肾病中，LCL衍生的DNA可能不适用于DNA甲基化研究。
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