Inoue S, Iimuro Y, Yamamoto M, Matsumoto Y, Sakihama T, Tasaka K
First Department of Surgery, Yamanashi Medical College, Japan.
Nihon Geka Gakkai Zasshi. 1991 Aug;92(8):913-20.
After injection of live E. coli, TNF in blood and culture supernatant of the isolated macrophages from the lung, liver, and spleen, were measured, and possible relationship between their TNF levels and lung edema was examined. The blood TNF activity increased significantly until 3h after the injection in the lethal group (p less than 0.01). The TNF activity of alveolar macrophages showed no changes even in the lethal group. The TNF activities of the liver and spleen macrophages decreased significantly in the lethal group (p less than 0.01-0.05), while those in the sublethal group that didn't induce lung edema and showed no significant decrease. The blood leukocyte count decreased until 6h after the injection in the both sublethal and lethal groups, but there was no significant difference between the two groups. The lung weight difference of the lethal group was significantly higher than that in the control group 12h after injection (p less than 0.05). Therefore, the elevated blood TNF activities in our study may be not elicited from alveolar macrophages but mainly from liver and spleen macrophages. There may be a relationship between the lung edema and the elevated blood TNF activity in the lethal groups.
注射活大肠杆菌后，检测血液以及从肺、肝和脾分离出的巨噬细胞培养上清液中的肿瘤坏死因子（TNF），并研究其TNF水平与肺水肿之间的可能关系。致死组在注射后3小时内血液TNF活性显著升高（p小于0.01）。即使在致死组，肺泡巨噬细胞的TNF活性也无变化。致死组肝和脾巨噬细胞的TNF活性显著降低（p小于0.01 - 0.05），而未诱发肺水肿的亚致死组则无显著降低。亚致死组和致死组在注射后6小时内血液白细胞计数均下降，但两组之间无显著差异。注射后12小时，致死组的肺重量差异显著高于对照组（p小于0.05）。因此，本研究中血液TNF活性升高可能并非源自肺泡巨噬细胞，而是主要来自肝和脾巨噬细胞。致死组中肺水肿与血液TNF活性升高之间可能存在关联。
