Morimoto H, Monden T, Miyoshi Y, Kawasaki Y, Nakanishi H, Shimano T, Mori T
Second Department of Surgery, Osaka University Medical School, Japan.
Nihon Geka Gakkai Zasshi. 1991 Sep;92(9):1094-7.
Abnormality of tumor suppressor gene p53 is supposed to be associated deeply with colon carcinogenesis. We have examined the aberrant expression of the p53 protein in human colorectal cancer or adenomatous tissues immunohistochemically using monoclonal antibody PAb 1801. In microwave-fixed colorectal tissues, p53 was successfully detected in more than 60% of carcinomas. Specific signal for p53 was restricted in the nuclei of cancer cells, while no staining was observed in adjacent normal mucosa. The incidence of p53 expression in colorectal carcinomas was not affected by pathological features such as tumor size, histological grade, nor depth of invasion. In about 10% of colorectal adenomas, weak signal was detected in a few adenomatous glands. Heat shock protein of 72 kDa (HSP72), known to form complex with the mutant-type of p53 in tumor cells, was also detected immunohistochemically in 25% of p53-positive cases. In these cases, high incidence of lymphnodal or distant metastasis was observed, which suggests that expression of both p53 and HSP72 may indicated biological malignancy of the colorectal carcinomas.
肿瘤抑制基因p53的异常被认为与结肠癌的发生密切相关。我们使用单克隆抗体PAb 1801通过免疫组织化学方法检测了人结直肠癌或腺瘤组织中p53蛋白的异常表达。在微波固定的结直肠组织中，超过60%的癌组织中成功检测到p53。p53的特异性信号局限于癌细胞的细胞核，而在相邻的正常黏膜中未观察到染色。结直肠癌中p53表达的发生率不受肿瘤大小、组织学分级或浸润深度等病理特征的影响。在约10%的结直肠腺瘤中，在少数腺瘤性腺体中检测到微弱信号。已知在肿瘤细胞中与突变型p53形成复合物的72 kDa热休克蛋白（HSP72），在25%的p53阳性病例中也通过免疫组织化学检测到。在这些病例中，观察到淋巴结或远处转移的高发生率，这表明p53和HSP72的表达可能表明结直肠癌的生物学恶性程度。
