用于蛋白质蘸笔纳米光刻的多孔多层涂层原子力显微镜探针

Porous multilayer-coated AFM tips for dip-pen nanolithography of proteins.

作者信息

Wu Chien-Ching, Xu Huaping, Otto Cees, Reinhoudt David N, Lammertink Rob G H, Huskens Jurriaan, Subramaniam Vinod, Velders Aldrik H

机构信息

Laboratory of Supramolecular Chemistry and Technology, MESA+ Institute for Nanotechnology and Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands.

出版信息

J Am Chem Soc. 2009 Jun 10;131(22):7526-7. doi: 10.1021/ja901756a.

DOI:10.1021/ja901756a

PMID:19441834

Abstract

A simple and novel method for fabricating nanoporous-structure-coated silicon nitride tips for dip-pen nanolithography (DPN) by using the layer-by-layer (LbL) technique has been developed. The pore sizes can be adjusted by treating the LbL films coated onto the amino-terminated self-assembled monolayer (NH(2)-SAM)-functionalized AFM tip surface with a base solution for different periods of time. This hydrophilic porous material can absorb biomolecules easily and also provides a larger-volume ink reservoir compared with a bare silicon nitride tip. Proof-of-concept of the porous AFM tip is demonstrated by using fluorescent proteins as ink molecules to fabricate protein patterns at the micrometer and submicrometer length scales.

摘要

已开发出一种简单且新颖的方法，通过使用层层（LbL）技术来制造用于蘸笔纳米光刻（DPN）的纳米多孔结构涂层氮化硅尖端。通过用碱溶液在不同时间段处理涂覆在氨基末端自组装单层（NH(2)-SAM）功能化原子力显微镜（AFM）尖端表面的LbL膜，可以调节孔径。这种亲水性多孔材料能够轻松吸收生物分子，并且与裸露的氮化硅尖端相比，还提供了更大体积的墨水储存器。通过使用荧光蛋白作为墨水分子，在微米和亚微米长度尺度上制造蛋白质图案，证明了多孔AFM尖端的概念验证。