Vazquez Susana, Acosta Nadia, Ruiz Didye, Calzada Naifi, Alvarez Angel M, Guzman Maria G
Pedro Kourí Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Havana City, Cuba.
J Virol Methods. 2009 Jul;159(1):6-9. doi: 10.1016/j.jviromet.2009.02.017. Epub 2009 Feb 24.
Using a serological test, different criteria have been established for classifying a case as primary or secondary dengue virus infection. Considering the dengue epidemiological situation in Cuba, IgG antibody response to dengue virus infection in serum samples from children and adults with a dengue 3 infection, in Havana city during the 2001-2002 epidemic was evaluated. Samples were collected on days 5-7 of fever onset and tested by an ELISA inhibition. A total of 713 serum samples positive for IgM antibody, 93 from children and 620 from adult patients were studied. Serum samples collected from healthy blood donors and patients not infected with dengue were included as controls. An IgG primary infection pattern was observed in sera collected from children, with titers of < or =20 in the 89.3% of the patients, while both, a primary and secondary patterns were observed in sera collected from adult patients with titers of < or =20 (13.4%) and > or =1280 (83.9%), respectively. These results permitted the definition of a primary or secondary case of dengue virus infection in serum samples collected during the acute phase of dengue virus infection.
使用血清学检测方法，已经建立了不同的标准来将病例分类为原发性或继发性登革病毒感染。考虑到古巴的登革热流行病学情况，对2001 - 2002年哈瓦那市登革热3型感染的儿童和成人血清样本中登革病毒感染的IgG抗体反应进行了评估。在发热开始的第5 - 7天采集样本，并通过ELISA抑制法进行检测。共研究了713份IgM抗体阳性的血清样本，其中93份来自儿童，620份来自成年患者。从健康献血者和未感染登革热的患者中采集的血清样本作为对照。在儿童采集的血清中观察到IgG原发性感染模式，89.3%的患者滴度≤20，而在成年患者采集的血清中观察到原发性和继发性模式，滴度≤20的患者占13.4%，滴度≥1280的患者占83.9%。这些结果有助于在登革病毒感染急性期采集的血清样本中定义原发性或继发性登革病毒感染病例。
