Lopez Job E, Porcella Stephen F, Schrumpf Merry E, Raffel Sandra J, Hammer Carl H, Zhao Ming, Robinson Mary Ann, Schwan Tom G
Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
Research Technologies Section, Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
Microbiology (Reading). 2009 Aug;155(Pt 8):2641-2651. doi: 10.1099/mic.0.029918-0. Epub 2009 May 14.
Borrelia hermsii is a blood-borne pathogen transmitted by the argasid tick Ornithodoros hermsi. Since spirochaete clearance in mice is associated with an IgM-mediated response, an immunoproteomic analysis was used to identify proteins reactive with IgM. We report that IgM from both mice and human patients infected with B. hermsii not only reacted with the previously identified variable membrane proteins but also identified candidate antigens including heat-shock proteins, an adhesin protein, ABC transporter proteins, flagellar proteins, housekeeping proteins, an immune evasion protein, and proteins with unknown function. Furthermore, IgM reactivity to recombinant glycerophosphodiester phosphodiesterase was detected during early spirochaete infection and prior to a detectable IgG response. Lastly, a conserved hypothetical protein was produced in Escherichia coli and tested with immune serum against B. hermsii and Borrelia recurrentis. These results identify a much larger set of immunoreactive proteins, and could help in the early serodiagnosis of this tick-borne infection.
赫氏疏螺旋体是一种通过钝缘蜱属的赫氏钝缘蜱传播的血源性病原体。由于小鼠体内螺旋体的清除与IgM介导的反应有关，因此采用免疫蛋白质组学分析来鉴定与IgM反应的蛋白质。我们报告称，感染赫氏疏螺旋体的小鼠和人类患者的IgM不仅与先前鉴定出的可变膜蛋白发生反应，还鉴定出了候选抗原，包括热休克蛋白、一种黏附蛋白、ABC转运蛋白、鞭毛蛋白、管家蛋白、一种免疫逃避蛋白以及功能未知的蛋白。此外，在螺旋体早期感染期间以及可检测到的IgG反应之前，检测到了IgM对重组甘油磷酸二酯磷酸二酯酶的反应性。最后，在大肠杆菌中产生了一种保守的假设蛋白，并用抗赫氏疏螺旋体和回归热疏螺旋体的免疫血清进行了检测。这些结果鉴定出了更多的免疫反应性蛋白，并可能有助于这种蜱传感染的早期血清学诊断。
