来自两种非光合细菌嗜热栖热菌的一种新型类胡萝卜素1,2-水合酶（CruF）。
A novel carotenoid 1,2-hydratase (CruF) from two species of the non-photosynthetic bacterium Deinococcus.
作者信息
Sun Zongtao, Shen Shaochuan, Wang Chao, Wang Hu, Hu Yaping, Jiao Jiandong, Ma Tingting, Tian Bing, Hua Yuejin
机构信息
Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, 310029 Hangzhou, China.
State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, 310032 Hangzhou, China.
出版信息
Microbiology (Reading). 2009 Aug;155(Pt 8):2775-2783. doi: 10.1099/mic.0.027623-0. Epub 2009 May 14.
A novel carotenoid 1,2-hydratase (CruF) responsible for the C-1',2' hydration of gamma-carotene was identified in the non-photosynthetic bacteria Deinococcus radiodurans R1 and Deinococcus geothermalis DSM 11300. Gene expression and disruption experiments demonstrated that dr0091 and dgeo2309 encode CruF in D. radiodurans and D. geothermalis, respectively. Their homologues were also found in the genomes of cyanobacteria, and exhibited little homology to the hydroxyneurosporene synthase (CrtC) proteins found mainly in photosynthetic bacteria. Phylogenetic analysis showed that CruF homologues form a separate family, which is evolutionarily distant from the known CrtC family.
在非光合细菌耐辐射奇异球菌R1和嗜热栖热放线菌DSM 11300中鉴定出一种新型类胡萝卜素1,2-水合酶（CruF），其负责γ-胡萝卜素的C-1',2'水合作用。基因表达和破坏实验表明，dr0091和dgeo2309分别在耐辐射奇异球菌和嗜热栖热放线菌中编码CruF。在蓝细菌基因组中也发现了它们的同源物，并且与主要在光合细菌中发现的羟基神经孢烯合酶（CrtC）蛋白几乎没有同源性。系统发育分析表明，CruF同源物形成一个独立的家族，在进化上与已知的CrtC家族相距甚远。
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