Tret'iak A G, Limarenko I M
Zh Evol Biokhim Fiziol. 1977 Jan-Feb;13(1):11-7.
The effect of acetylcholine (ACh) and cyclic 3',5'-adenosine monophosphate (cAMP) on phosphoinositide (PI) metabolism and associated changes of the permeability of the nervous fibers in the crustaceans Carcinum maenas and Eriphia spinifrons, as well as in the frog Rana temporaria, has been investigated. It was shown that ACh induces a significant decrease in the content of triphosphoinositides (TPI) in the nervous fibers of the crabs and increases their potassium permeability, which in its turn results in depolarization of the fibers. ACh did not affect frog nervous fibers. cAMP significantly increases the incorporation of 32P into TPI fraction of crab nervous fibers during conduction of excitation and also facilitates repolarization of the fibers after application of ACh. Proserine effectively protects nervous fibers from the influence of ACh, indicating possible participation of acetylcholinesterase in TPI hydrolysis. The data obtained suggest that PI are involved into the regulation of membrane permeability of crab nervous fibers to potassium ions.
研究了乙酰胆碱（ACh）和环磷酸腺苷（cAMP）对海螯虾（Carcinum maenas）、刺螯虾（Eriphia spinifrons）以及林蛙（Rana temporaria）神经纤维中磷酸肌醇（PI）代谢的影响以及相关神经纤维通透性的变化。结果表明，ACh可使蟹类神经纤维中三磷酸肌醇（TPI）含量显著降低，并增加其钾离子通透性，进而导致纤维去极化。ACh对蛙类神经纤维无影响。cAMP在兴奋传导过程中可显著增加32P掺入蟹类神经纤维的TPI组分中，并且在施加ACh后还促进纤维复极化。毒扁豆碱可有效保护神经纤维免受ACh的影响，表明乙酰胆碱酯酶可能参与TPI水解。所得数据表明，PI参与蟹类神经纤维对钾离子的膜通透性调节。
