Geng Yi-Jie, Gao Shi-Tong, Huang Da-Na, Zhao Yi-Rui, Liu Jian-ping, Li Xiao-Heng, Zhang Ren-Li
Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518020, People's Republic of China.
Parasitol Res. 2009 Sep;105(3):843-51. doi: 10.1007/s00436-009-1470-5. Epub 2009 May 15.
The aim of the present study was to identify sex-specific genes in adult Anopheles anthropophagus. As the major malaria vector and Brugia malayi vector in the Asian continent, female Anopheles mosquitoes take blood meals and transmit pathogens through this pathway, while males are nectar feeders. This complex behavior is controlled at several levels, but is probably initiated by the genetic background difference between these two groups. In our study, a subtractive cDNA library for female A. anthropophagus was constructed using the suppression subtractive hybridization (SSH) technique and then 3,074 clones from the female SSH library were analyzed using a microarray-based survey. Genes that were expressed differentially according to sex in A. anthropophagus were screened using real-time polymerase chain reaction and reverse transcription polymerase chain reaction. In our results, we report a series of genes which may be involved in female-specific mosquito behavior, including an inorganic phosphate transporter, a serine protease, the salivary protein GP35-2, and the D7 cluster salivary protein. These findings will provide clues to the nature of insect vectors and open up unprecedented opportunities to develop novel strategies for the control of mosquito-borne diseases.
本研究的目的是鉴定成年嗜人按蚊中的性别特异性基因。作为亚洲大陆主要的疟疾传播媒介和马来丝虫传播媒介，雌性按蚊吸食血液并通过此途径传播病原体，而雄性则以花蜜为食。这种复杂的行为在多个层面受到控制，但可能是由这两组之间的遗传背景差异引发的。在我们的研究中，利用抑制性消减杂交（SSH）技术构建了雌性嗜人按蚊的消减cDNA文库，然后使用基于微阵列的调查分析了来自雌性SSH文库的3074个克隆。利用实时聚合酶链反应和逆转录聚合酶链反应筛选出在嗜人按蚊中根据性别差异表达的基因。在我们的研究结果中，我们报告了一系列可能参与雌性特异性蚊子行为的基因，包括一种无机磷酸盐转运蛋白、一种丝氨酸蛋白酶、唾液蛋白GP35-2和D7簇唾液蛋白。这些发现将为昆虫媒介的本质提供线索，并为开发控制蚊媒疾病的新策略开辟前所未有的机会。
