Dietel M, Dorn G, Montz R, Altenähr E
Acta Endocrinol (Copenh). 1977 Jul;85(3):541-7. doi: 10.1530/acta.0.0850541.
The effect of different calcium concentrations as well as dibutyryl-cyclic adenosine 3',5'-monophosphate (DB-cAMP) on the secretion of parathyroid hormone by human parathyroid adenomas taken from patients with primary hyperparathyroidism (pHPT) was studied in organ culture. Their influence on the release of hormone was determined. The tissue was incubated in culture medium for 4 h; the medium was changed hourly and analyzed for immunoreactive parathyroid hormone (PTH) by radioimmunoassay. The hormone secretion showed an inverse relationship to different calcium concentrations in the medium and could be stimulated independently of the calcium concentration by adding DB-cAMP. These results suggest that the examined parathyroid adenomas are sensitive to physiological stimuli.
在器官培养中研究了不同钙浓度以及二丁酰环磷腺苷3',5'-单磷酸（DB - cAMP）对取自原发性甲状旁腺功能亢进症（pHPT）患者的人甲状旁腺腺瘤分泌甲状旁腺激素的影响。确定了它们对激素释放的影响。将组织在培养基中孵育4小时；每小时更换培养基，并通过放射免疫测定法分析免疫反应性甲状旁腺激素（PTH）。激素分泌与培养基中不同钙浓度呈负相关，并且通过添加DB - cAMP可独立于钙浓度进行刺激。这些结果表明所检测的甲状旁腺腺瘤对生理刺激敏感。
