Fusion of images in multistep analysis of neovascularisation.

PURPOSE

The aim of our study was to develop a method for the fusion of images received after repeated staining of the same sample taking into account spatial differences between the images.

MATERIAL AND METHODS

A method of objective fusion performance was investigated on the images receiving during multistep staining of the xenograft tumour cross-sections.

RESULTS

It was shown that several images receiving from different steps of staining procedures may be successfully fused by fluorescent marking of slide position with Trout red blood cells before analysis.

CONCLUSIONS

Proposed technique provides an accurate rigid fusion of light and fluorescent images receiving during multistep image analysis under microscope and may be applied for study of neovascularisation.