Chen Nan-nan, Zhang Ge-ying, Xiang Yang, Zhang Li, Zhang De-jie, Huang Shi-lin
Department of Traditional Chinese Medicine and hematology, 210th Hospital of PLA, Dalian 116021, China.
Zhong Yao Cai. 2009 Jan;32(1):78-82.
To explore the effects of psoralen (PSO) and long wave ultraviolet A (PUVA) on expression of Fas and FasL in apoptosis of NB4 and K562 leukemia cells.
The NB4 and K562 cells were taken as the study objects and their apoptosis ratios, ultrastructure changes and the expression of Fas and FasL were detected in order to observe the effects of PSO and UVA of wave length 360 nm on human leukemia cells. The factorial design and analysis of variance were used to analyze the interaction among the factors.
All of PSO, UVA and PUVA could induce the apoptosis of NB4 and K562 cells, and the effects of PUVA were stronger than the other two. After treated with PUVA, the NB4 and K562 cells all showed obvious ultrastruture changes about apoptsis under the electron microscope. All of PSO, UVA and PUVA could increase the expression of Fas gene and protein, and decrease the expression of FasL gene and protein. Moreover, the effects of PUVA were stronger than the other two.
PUVA can induce the apoptosis of NB4 and K562 cells and the effects are the strongest, one of the pathway of PUVA to induce apoptosis is to upregulate the expression of Fas gene and downregulate the expression of FasL gene.
探讨补骨脂素（PSO）及长波紫外线A（PUVA）对NB4和K562白血病细胞凋亡中Fas和FasL表达的影响。
以NB4和K562细胞为研究对象，检测其凋亡率、超微结构变化及Fas和FasL的表达，以观察PSO及波长360nm的紫外线A（UVA）对人白血病细胞的影响。采用析因设计及方差分析来分析各因素间的交互作用。
PSO、UVA及PUVA均可诱导NB4和K562细胞凋亡，且PUVA的作用强于其他两者。经PUVA处理后，NB4和K562细胞在电镜下均呈现出明显的凋亡超微结构变化。PSO、UVA及PUVA均可增加Fas基因和蛋白的表达，降低FasL基因和蛋白的表达。此外，PUVA的作用强于其他两者。
PUVA可诱导NB4和K562细胞凋亡且作用最强，PUVA诱导凋亡的途径之一是上调Fas基因的表达并下调FasL基因的表达。
