[从蒙古族儿童中筛选与鉴定益生菌双歧杆菌]

[Screening and identification of probiotic bifidobacterium from Mongolian children].

作者信息

Gao Pengfei, Sun Zhihong, Ma Shiwei, Wang Qiushi, Gao Jie, Deng Cengyuan, Zhang Heping

机构信息

Key Lab of Dairy Biotechnology and Bioengineer, Education Ministry of China, Inner Mongolia Agricultural University, Huhhot 010018, China.

出版信息

Wei Sheng Wu Xue Bao. 2009 Feb;49(2):210-6.

PMID:19445177

Abstract

OBJECTIVE

Bifidobacterium has important physiological activity in gastrointestinal tract of human and animals, and the tolerability of acid, intestinal juice and oxgall bile salt are the key factors that influence the function of living bacteria preparation. In this study, feces of 12 healthy Mongolian children were selected to isolate Bifidobacterium with probiotic properties.

METHODS

Bifidobacterium was isolated from feces by selective medium and identified by physiological biochemical test. The tolerability of acid, intestinal juice and oxgall bile salt of 11 Bfidobacteria were studied and B. animalis V9 was identified further by molecular biological approach 16S rDNA.

RESULTS

Eleven Bifidobacteria isolated from feces of 12 healthy Mongolian children were identified as B. adolescentis (A1, H3, G4, A8 and V10), B. longum (C6, C7 and D11), B. pseudocatenlatum (B2), B. bifidum (G5), B. animalis (V9). B. animalis V9 had the best acid tolerance with the survival rate 92.4% in artificial gastric juice at pH2.0 for 3h, whereas others had lower than 31.25%. B. animalis V9 also had good survival rate (99.7%) in artificial intestinal juice at pH8.0 for 8h after anaerobic cultured 3h in artificial gastric juice at pH2.0, and tolerated oxgall bile salt at concentration of 0.3%. B. animalis V9 was identified further by molecular biological approach 16S rDNA and result showed the homologies of B. animalis V9 was 99% with Bifidobacterium animalis subsp. lactic BB12.

CONCLUSION

B. animalis V9 had good probiotic properties to be potentiallyused in dairy products and health products.

摘要

目的

双歧杆菌在人和动物胃肠道中具有重要的生理活性，其对酸、肠液及牛磺胆盐的耐受性是影响活菌制剂功能的关键因素。本研究选取12名健康蒙古族儿童粪便分离具有益生菌特性的双歧杆菌。

方法

采用选择性培养基从粪便中分离双歧杆菌，通过生理生化试验进行鉴定。研究11株双歧杆菌对酸、肠液及牛磺胆盐的耐受性，并采用分子生物学方法16S rDNA对动物双歧杆菌V9进行进一步鉴定。

结果

从12名健康蒙古族儿童粪便中分离出的11株双歧杆菌分别鉴定为青春双歧杆菌（A1、H3、G4、A8和V10）、长双歧杆菌（C6、C7和D11）、假链状双歧杆菌（B2）、两歧双歧杆菌（G5）、动物双歧杆菌（V9）。动物双歧杆菌V9具有最佳的耐酸性，在pH2.0人工胃液中3小时存活率为92.4%，而其他菌株低于31.25%。动物双歧杆菌V9在pH2.0人工胃液中厌氧培养3小时后，在pH8.0人工肠液中8小时也具有良好的存活率（99.7%），并能耐受0.3%浓度的牛磺胆盐。采用分子生物学方法16S rDNA对动物双歧杆菌V9进行进一步鉴定，结果显示动物双歧杆菌V9与动物双歧杆菌亚种乳酸BB12的同源性为99%。