Kwon Hyuk-Sang, Yang Eun-Hee, Lee Seung-Hun, Yeon Seung-Woo, Kang Byung-Hwa, Kim Tae-Yong
ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd., Biotechnology Laboratory, 260-5, Eonnam-ri, Kuseong-eup, Yongin, Gyongki-do 449-915, Korea.
FEMS Microbiol Lett. 2005 Sep 1;250(1):55-62. doi: 10.1016/j.femsle.2005.06.041.
This study aimed at developing a novel multiplex polymerase chain reaction (PCR) primer set for identification of the potentially probiotic Bifidobacterium species B. adolescentis, B. animalis subsp. animalis (B. animalis), B. bifidum, B. breve, B. longum biovar infantis (B. infantis), B. animalis subsp. lactis B. lactis, B. longum biovar longum (B. longum) and B. pseudolongum. The primer set comprised specific and conserved primers and was derived from the integrated sequences of 16S and 23S rRNA genes and the rRNA intergenic spacer region (ISR) of each species. It could detect and identify type strains and isolates from pharmaceuticals or dairy products corresponding to the eight Bifidobacterium species with high specificity. It was also useful for screening of the related strains from natural sources such as the gastro-intestinal tract and feces. We suggest that the assay system from this study is an efficient tool for simple, rapid and reliable identification of Bifidobacterium species for which probiotic strains are known.
本研究旨在开发一种新型多重聚合酶链反应（PCR）引物组，用于鉴定具有潜在益生菌特性的双歧杆菌属物种，包括青春双歧杆菌、动物双歧杆菌动物亚种（动物双歧杆菌）、两歧双歧杆菌、短双歧杆菌、长双歧杆菌婴儿亚种（婴儿双歧杆菌）、动物双歧杆菌乳酸亚种（乳酸双歧杆菌）、长双歧杆菌长亚种（长双歧杆菌）和假长双歧杆菌。该引物组由特异性和保守性引物组成，来源于每个物种的16S和23S rRNA基因以及rRNA基因间隔区（ISR）的整合序列。它能够以高特异性检测和鉴定与这八种双歧杆菌物种相对应的模式菌株以及来自药品或乳制品的分离株。它也有助于从胃肠道和粪便等天然来源中筛选相关菌株。我们认为，本研究中的检测系统是一种用于简单、快速且可靠地鉴定已知具有益生菌菌株的双歧杆菌物种的有效工具。
