Chen Bin, Ma Chao, Zhou Shining, Bu Haitao, Jiang Siping, Li Hui
Tibetan Plateau Institute of Biology, Lhasa 850001, China.
Wei Sheng Wu Xue Bao. 2009 Feb;49(2):217-26.
To study the bacterial diversity in sediment and water from two disused thermal vents in Yangbajing, Tibet, China.
We constructed 16S rRNA gene libraries of the total DNA from three samples. Sediment sample A and water sample A were from thermal vent A. Sediment sample B was from thermal vent B. Positive clones from the libraries were analyzed randomly by amplified ribosomal DNA restriction analysis. Positive clones' sequences of every Operational Taxonomic Units from libraries were determined, and sequence data were submitted to GenBank and contrasted to those known sequences. Phylogenetic trees were built up by using MEGA4.0 program.
Most of bacteria communities of the two thermal vents were typical thermophilc inhabitants, the predominant proteobacteria communities were found in thermal vent A and thermal vent B (ratios in sediment A, water sample A and sediment B were 41.08%, 38.00% and 42.57%) One of the sub-predominant bacteria communities, Deinococcus-Thermus, was found in thermal vent A and thermal vent B (ratios in sediment A, water sample A and sediment B were 10.71%, 20.00% and 21.30%). Moreover, one of the sub-predominant bacteria communities, Acidobacteria that was rarely found in hot springs or thermal vents, was present both in sediments of thermal vent A and B (16.07%, 19.15%). The third sub-predominant community in sediment of thermal vent A was Eubacterium sp. (14.28%), belonging to phylum Firmicutes whereas no Acidobacteria was found in water sample A. Instead, Hydrogenobacter, belonging to phylum Aquificae, was another sub-predominant community (16.00%) in water sample A. In addition, Chloroflexi, Cyanobacteria and CFB group ( Cytophaga-Flexibacter-Bacteroides) were detected in both thermal vents.
Compared with and contrasted to the references, we found that bacteria communities in Yangbajing thermal field were similar to that in some hot springs and thermal vents around the world but slightly abnormal. Some bacteria communities in thermal vent A and thermal vent B were not very popular, such as Vibrio sp., Bacteriovorax sp., HolophagalAcidobacterium, Verrucomicrobium.
研究中国西藏羊八井两个废弃热液喷口沉积物和水体中的细菌多样性。
构建了来自三个样本总DNA的16S rRNA基因文库。沉积物样本A和水样A来自热液喷口A。沉积物样本B来自热液喷口B。通过扩增核糖体DNA限制性分析对文库中的阳性克隆进行随机分析。测定文库中每个操作分类单元阳性克隆的序列，并将序列数据提交至GenBank并与已知序列进行对比。使用MEGA4.0程序构建系统发育树。
两个热液喷口的大多数细菌群落是典型的嗜热菌，在热液喷口A和热液喷口B中发现了主要的变形菌群落（在沉积物A、水样A和沉积物B中的比例分别为41.08%、38.00%和42.57%）。次优势细菌群落之一，嗜热栖热放线菌，在热液喷口A和热液喷口B中被发现（在沉积物A、水样A和沉积物B中的比例分别为10.71%、20.00%和21.30%）。此外，次优势细菌群落之一，在温泉或热液喷口中很少发现的酸杆菌，在热液喷口A和B的沉积物中均有存在（分别为16.07%、19.15%）。热液喷口A沉积物中的第三个次优势群落是真细菌属（14.28%），属于厚壁菌门，而在水样A中未发现酸杆菌。相反，属于产水菌门的氢杆菌是水样A中的另一个次优势群落（16.00%）。此外，在两个热液喷口中均检测到绿弯菌门、蓝细菌和CFB组（噬纤维菌-屈挠杆菌-拟杆菌）。
与参考文献进行比较和对比后，我们发现羊八井热田的细菌群落与世界上一些温泉和热液喷口的细菌群落相似，但略有异常。热液喷口A和热液喷口B中的一些细菌群落不太常见，如弧菌属、食菌蛭弧菌、全噬酸杆菌、疣微菌门。
