Sreedhar Balamurali, Damtew Andualem, Seidel-Morgenstern Andreas
Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
J Chromatogr A. 2009 Jun 19;1216(25):4976-88. doi: 10.1016/j.chroma.2009.04.057. Epub 2009 May 3.
Complementing classical isocratic elution, several more sophisticated operating modes have been proposed and are applied in preparative chromatography in order to improve performance. One such approach is gradient elution, where the solvent strength is altered by varying the fraction of a modifier added to the mobile phase to enhance selectivity and to achieve faster elution. Another useful technique is closed-loop recycling, allowing better peak resolution and increased yields. This study focuses on a modified new scheme which incorporates the advantages of both gradient elution and closed-loop recycling for the separation of a ternary mixture where the intermediately eluting component is the target. A parametric study was carried out using typical adsorption isotherm parameters to elucidate the effects of varying loading factors and parameters specific to the two basic operational modes on production rates and yields. A comparison was also made between the proposed scheme and conventional techniques. It was found that the studied scheme could exploit increased column loadings and offers significantly higher production rates.
作为经典等度洗脱的补充，人们提出了几种更复杂的操作模式，并应用于制备色谱中以提高性能。一种这样的方法是梯度洗脱，通过改变添加到流动相中的改性剂的比例来改变溶剂强度，以提高选择性并实现更快的洗脱。另一种有用的技术是闭环循环，可实现更好的峰分离度并提高产率。本研究聚焦于一种改进的新方案，该方案结合了梯度洗脱和闭环循环的优点，用于分离三元混合物，其中中间洗脱的组分是目标物。使用典型的吸附等温线参数进行了参数研究，以阐明不同负载因子和两种基本操作模式特有的参数对生产率和产率的影响。还对所提出的方案与传统技术进行了比较。结果发现，所研究的方案可以利用增加的柱负载量，并提供显著更高的生产率。
