Polkowska J, Bérault A, Hurbain-Kosmath I, Jolly G, Jutisz M
Laboratoire des Hormones Polypeptidiques, CNRS, Gif-sur-Yvette, France.
Neuroendocrinology. 1991 Sep;54(3):267-73. doi: 10.1159/000125885.
Using single hormone-staining immunocytochemical methods, we have recently characterized a novel cell line, RC-4B/C, established from an aged male rat pituitary adenoma. This cell line contained all known anterior pituitary cell types including gonadotropes. Gonadotropin-releasing hormone receptors were also present. A recent re-examination of the cell types using single hormone stains showed that the cell line underwent an alteration in the percentage of different cell types as compared to the data obtained 2 years ago. The proportion of follicle-stimulating hormone-beta (FSH beta), luteinizing hormone-beta (LH beta), prolactin (PRL), and adrenocorticotropic hormone cells increased significantly (p less than or equal to 0.001), while the proportion of growth hormone (GH), and thyrotropin-beta cells did not change. Dual staining of the monolayers showed that the cell line contained many bihormonal cells producing FSH beta + LH beta, FSH beta + PRL and LH beta + PRL. The proportion of bihormonal FSH beta + LH beta and FSH beta + PRL cells was preponderant over monohormonal cells, while the proportion of bihormonal LH beta + PRL cells was in the same range as that of monohormonal cells. Preliminary data with dual labeling also revealed the presence of GH and PRL in the same cell, but complete absence of a combination such as FSH beta + GH. No search for the presence of other bihormonal or multihormonal cells was performed. In short, our data show that the majority of the cells in the cell line RC-4B/C contain FSH beta, LH beta and PRL and that among these cells many bihormonal cells are present.
利用单激素染色免疫细胞化学方法，我们最近鉴定了一种新的细胞系RC-4B/C，它是从一只老年雄性大鼠垂体腺瘤中建立的。该细胞系包含所有已知的垂体前叶细胞类型，包括促性腺激素细胞。促性腺激素释放激素受体也存在。最近使用单激素染色对细胞类型进行的重新检查表明，与两年前获得的数据相比，该细胞系中不同细胞类型的百分比发生了变化。促卵泡激素β（FSHβ）、促黄体生成素β（LHβ）、催乳素（PRL）和促肾上腺皮质激素细胞的比例显著增加（p≤0.001），而生长激素（GH）和促甲状腺激素β细胞的比例没有变化。单层细胞的双重染色显示，该细胞系包含许多产生FSHβ+LHβ、FSHβ+PRL和LHβ+PRL的双激素细胞。双激素FSHβ+LHβ和FSHβ+PRL细胞的比例高于单激素细胞，而双激素LHβ+PRL细胞的比例与单激素细胞的比例相同。双重标记的初步数据还显示同一细胞中存在GH和PRL，但完全不存在FSHβ+GH等组合。未对其他双激素或多激素细胞的存在进行搜索。简而言之，我们的数据表明，细胞系RC-4B/C中的大多数细胞含有FSHβ、LHβ和PRL，并且在这些细胞中有许多双激素细胞。
