Ivanov Iuri B, Gritsenko Viktor A, Kuzmin Michael D
Department of Human Microbiology, Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Orenburg 460056, Russia.
Asian J Androl. 2009 Jul;11(4):517-20. doi: 10.1038/aja.2009.24. Epub 2009 May 18.
We compared the potential phenotypic properties of coryneform bacteria associated with chronic prostatitis syndrome (CPS), such as secretory inhibitor of lysozyme (SIL) and secretory inhibitor of platelet microbicidal protein (SIPMP). A total of 110 clinical isolates of coryneform bacteria isolated from the seminal fluid of healthy men and men with CPS were tested. SIPMP production was tested by inhibiting platelet microbicidal protein (PMP) bioactivity against Bacillus subtilis, and was expressed as percentage of inhibition of PMP bactericidal activity. SIL production was tested by inhibiting lysozyme activity against Micrococcus lysodeikticus and was expressed in microgram per millilitre of inactivated lysozyme. A significantly higher proportion of CPS strains (58.7% vs. 19.2 %) was SIPMP-positive compared with non-CPS strains (P < 0.01). Of the CPS strains tested, 77.8% were SIL-positive compared with 34% of the non-CPS isolates (P < 0.05). These results suggest that the diagnosis of CPS should not rely solely on classical parameters, for example, the identification and counting of microorganisms, but the functional significance of these parameters must be estimated, possibly by the concentration of different bacterial substrains, detection of opportunistic microorganisms with pathogenic properties, such as pronounced resistance to the cationic antimicrobial peptides, and/or the ability to inhibit the antimicrobial host defence factors.
我们比较了与慢性前列腺炎综合征（CPS）相关的棒状杆菌的潜在表型特性，如溶菌酶分泌抑制剂（SIL）和血小板杀菌蛋白分泌抑制剂（SIPMP）。对从健康男性和患有CPS的男性精液中分离出的110株棒状杆菌临床分离株进行了检测。通过抑制血小板杀菌蛋白（PMP）对枯草芽孢杆菌的生物活性来检测SIPMP的产生，并以PMP杀菌活性的抑制百分比表示。通过抑制溶菌酶对溶壁微球菌的活性来检测SIL的产生，并以每毫升失活溶菌酶的微克数表示。与非CPS菌株相比，CPS菌株中SIPMP阳性的比例显著更高（58.7%对19.2%，P<0.01）。在检测的CPS菌株中，77.8%为SIL阳性，而非CPS分离株中这一比例为34%（P<0.05）。这些结果表明，CPS的诊断不应仅依赖于经典参数，例如微生物的鉴定和计数，还必须评估这些参数的功能意义，可能通过不同细菌亚菌株的浓度、检测具有致病特性的机会性微生物（如对阳离子抗菌肽有明显抗性）和/或抑制抗菌宿主防御因子的能力来评估。
