Shen Mei, Gao Jing, Li Jianan, Su Juan
Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Can J Physiol Pharmacol. 2009 May;87(5):396-401. doi: 10.1139/y09-007.
To examine the comparative effects of different frequencies of electrical stimulation (ES) on angiogenesis and gene expression, New Zealand white rabbits with femoral artery ligation in one hindlimb and electrode implantation on the sciatic nerve of the same hindlimb were randomly assigned to 4 groups: control group, 1 Hz group, 10 Hz group, and 40 Hz group. The ES procedure involved 5 min stimulation, followed by 5 min rest, repeated 8 times daily for 4 consecutive weeks. The resting blood flow (RBF) was measured via the microspheres technique. Collateralization was evaluated by immunohistochemistry. Angiogenic factors were analyzed by real-time RT-PCR. Both RBF and capillary density were significantly increased in the 10 Hz and 40 Hz groups, but were not changed in the 1 Hz group. Vascular endothelial growth factor (VEGF) mRNA was highest in the 40 Hz group. Hypoxia-inducible factor 1alpha (HIF-1alpha) mRNA was significantly elevated only in the 40 Hz group. VEGF receptor fetal liver kinase 1 (Flk-1) mRNA was upregulated equally in the 10 Hz and 40 Hz groups, but fibroblast growth factor 2 (FGF-2) mRNA did not change in any group. Our results suggest that the optimal frequency of ES for angiogenesis is within the 10-40 Hz range.
为研究不同频率电刺激（ES）对血管生成和基因表达的比较效应，将一侧后肢股动脉结扎并在同侧后肢坐骨神经植入电极的新西兰白兔随机分为4组：对照组、1Hz组、10Hz组和40Hz组。电刺激程序包括5分钟刺激，随后休息5分钟，每天重复8次，连续进行4周。通过微球技术测量静息血流量（RBF）。采用免疫组织化学法评估侧支循环。通过实时逆转录聚合酶链反应分析血管生成因子。10Hz组和40Hz组的RBF和毛细血管密度均显著增加，但1Hz组未发生变化。血管内皮生长因子（VEGF）mRNA在40Hz组中最高。低氧诱导因子1α（HIF-1α）mRNA仅在40Hz组中显著升高。VEGF受体胎儿肝激酶1（Flk-1）mRNA在10Hz组和40Hz组中均上调，但成纤维细胞生长因子2（FGF-2）mRNA在任何组中均未改变。我们的结果表明，促进血管生成的最佳电刺激频率在10-40Hz范围内。
