Sun Guangda, Su Zhongyi, Chen Meng, Yuan Dongxing
(Environmental Science Research Center, Xiamen University, Xiamen 361005, China.
Se Pu. 2009 Jan;27(1):54-8.
Using HLB cartridge for extraction and cleanup, a method for simultaneous determination of 4 tetracycline and 6 quinolone antibiotics in environmental water samples was developed by ultra pressure liquid chromatography coupled with tandem mass spectrometry. Using river water and sea water as matrices and carbadox as surrogate, the recoveries and relative standard deviations (RSD, n = 4) were 94.0% - 117.0% and 2.0% - 9.7% for 4 tetracyclines at 20.0 ng/L and 100.0 ng/L spiking levels and 63.6% - 93.9% and 1.6% - 8.1% for 6 quinolones at 5.0 ng/L and 20.0 ng/L spiking levels, respectively. The detection limits were 20.0 ng/L for 4 tetracyclines and 0.4 ng/L for 6 quinolones. The method has been successfully applied to the survey of 10 target antibiotic residues in Jiulong River estuary, Fujian.
采用HLB柱进行萃取和净化，建立了一种超高效液相色谱-串联质谱法同时测定环境水样中4种四环素类和6种喹诺酮类抗生素的方法。以河水和海水为基质，以卡巴氧为替代物，在20.0 ng/L和100.0 ng/L加标水平下，4种四环素类抗生素的回收率和相对标准偏差（RSD，n = 4）分别为94.0% - 117.0%和2.0% - 9.7%；在5.0 ng/L和20.0 ng/L加标水平下，6种喹诺酮类抗生素的回收率和相对标准偏差分别为63.6% - 93.9%和1.6% - 8.1%。4种四环素类抗生素的检出限为20.0 ng/L，6种喹诺酮类抗生素的检出限为0.4 ng/L。该方法已成功应用于福建九龙江口10种目标抗生素残留的检测。
