Lewis L C, Bruck D J, Sumerford D V, Gunnarson R D
USDA-ARS, Corn Insects and Crop Genetics Research Unit, Genetics Laboratory, Ames, IA 50011, USA.
J Econ Entomol. 2009 Apr;102(2):624-8. doi: 10.1603/029.102.0221.
Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) is one of the most damaging insect pests of corn. Studies were conducted to determine whether live larval counts obtained from corn whorls were predictive of the amount of larval tunneling that would result in the stalk of the plant 40 d later at the end of larval development. Whorls from plants treated with Dipel 10G (6,400 IU per whorl) and untreated controls, both infested with O. nubilalis neonates, were evaluated for the number of live larvae in 50 whorls 5, 7, 9, and 12 d after Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) application. Forty days after larval infestation, 25 plants from each plot were split from tassel to base, and the length of larval tunneling was recorded. There was a strong relationship between numbers of live larvae in the plant whorl and the length of larval tunneling that resulted. While linear at each location, there was significant variation in the relationship among locations, indicating that comparisons could not be made between years or locations within a year. Blocks within a location on a given year did not vary significantly and reproducible results were obtained each year within a given location as well as on any of the whorl pulling dates evaluated. Because of its ease of use, predictability, and rapid return of results, we propose this technique as an additional method to shot-hole feeding and stalk splitting to evaluate the effectiveness of O. nubilalis management strategies.
欧洲玉米螟(Ostrinia nubilalis (Hübner),鳞翅目:草螟科)是玉米最具危害性的害虫之一。开展了多项研究,以确定从玉米心叶中获得的活幼虫数量是否可预测幼虫发育末期40天后植株茎秆中幼虫蛀道的数量。用敌宝10G(每心叶6400国际单位)处理的植株的心叶和未处理的对照的心叶,均接种欧洲玉米螟初孵幼虫,在施用苏云金芽孢杆菌(Berliner)(芽孢杆菌目:芽孢杆菌科)后5、7、9和12天,对50个心叶中的活幼虫数量进行评估。幼虫侵染40天后,将每个小区的25株植株从穗部到基部劈开,记录幼虫蛀道的长度。植株心叶中的活幼虫数量与由此产生的幼虫蛀道长度之间存在很强的相关性。虽然在每个地点呈线性关系,但不同地点之间的关系存在显著差异,这表明不同年份或同一年份内不同地点之间无法进行比较。给定年份内一个地点的区组没有显著差异,并且在给定地点以及所评估的任何心叶取样日期每年都能获得可重复的结果。由于其使用方便、可预测性强且结果回报迅速,我们建议将该技术作为一种额外的方法,与蛀孔取食法和茎秆劈开法一起,用于评估欧洲玉米螟管理策略的有效性。