Li Yaoyao, Shen Yuemao, Zhu Xiangcheng, Du Liangcheng
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
J Nat Prod. 2009 Jul;72(7):1328-30. doi: 10.1021/np900193j.
Fumonisins and AAL toxins are polyketide-derived mycotoxins produced by several important fungal pathogens of crop plants. The toxins contain a linear, dimethylated polyketide chain, but differ in chain length and methylation positions. Here, we have isolated the major metabolites from a fumonisin null mutant, Fusarium verticillioides strain 5777, that has been transformed with the AAL-toxin polyketide synthase gene (ALT1). The results showed that the metabolites maintained the chain length and methylation pattern of fumonisins. This suggests that the timing and regioselectivity of the methyltransferase of the ALT1 PKS could be reprogrammed in this Fusarium transformant.
伏马毒素和AAL毒素是由几种重要的农作物真菌病原体产生的聚酮化合物衍生的霉菌毒素。这些毒素含有一条线性的、二甲基化的聚酮链，但链长和甲基化位置不同。在这里，我们从一个伏马毒素缺失突变体——轮枝镰孢菌5777菌株中分离出了主要代谢产物，该菌株已被转入AAL毒素聚酮合酶基因（ALT1）。结果表明，这些代谢产物保持了伏马毒素的链长和甲基化模式。这表明，在这个镰刀菌转化体中，ALT1聚酮合酶甲基转移酶的时间和区域选择性可以被重新编程。
