Agarwal S, Reynolds M A, Pou S, Peterson D E, Charon J A, Suzuki J B
Department of Periodontics, University of Maryland Dental School, Baltimore.
Oral Microbiol Immunol. 1991 Feb;6(1):51-61. doi: 10.1111/j.1399-302x.1991.tb00451.x.
Human polymorphonuclear cell (PMN) viability, morphology, adherence, chemotaxis, oxidative metabolism, degranulation and phagocytosis were evaluated following treatment with sanguinarine (SANG). SANG was noncytotoxic to PMNs at all concentrations tested (0.31-200 microM). SANG entered the PMNs rapidly without altering the membrane fluidity and localized in the nuclear matrix. SANG (1.56-6.21 microM) inhibited chemotaxis, chemokinesis and adhesion in a dose-dependent manner, with a complete inhibition at 6.2 microM concentration. Concentrations of SANG up to 1.56 microM did not affect PMN oxidative burst; however, higher concentrations were found to inhibit basal as well as PMA-induced superoxide anion generation. The effect of SANG was time- and dose-dependent, and could be reversed if the PMNs were exposed to 12.5 microM or lower concentrations of SANG for less than 5 min. Autologous serum increased the tolerance of PMNs to SANG. Exogenous Ca2+ or Mg2+ did not alter the SANG-mediated inhibition of PMN functions. Treatment of PMNs with 3.12 microM or higher concentrations of SANG also resulted in inhibition of PMN degranulation and phagocytosis. The results suggest that SANG-mediated inhibition of PMN functions, without cytolysis or resultant release of inflammatory mediators, may have clinical implications.
在用血根碱（SANG）处理后，评估了人类多形核细胞（PMN）的活力、形态、黏附、趋化性、氧化代谢、脱颗粒和吞噬作用。在所有测试浓度（0.31 - 200微摩尔）下，SANG对PMN均无细胞毒性。SANG迅速进入PMN，未改变膜流动性，并定位于核基质中。SANG（1.56 - 6.21微摩尔）以剂量依赖性方式抑制趋化性、趋化运动和黏附，在6.2微摩尔浓度时完全抑制。高达1.56微摩尔的SANG浓度不影响PMN的氧化爆发；然而，发现较高浓度会抑制基础以及佛波酯（PMA）诱导的超氧阴离子生成。SANG的作用具有时间和剂量依赖性，如果PMN暴露于12.5微摩尔或更低浓度的SANG少于5分钟，其作用可逆转。自体血清增加了PMN对SANG的耐受性。外源性Ca2+或Mg2+未改变SANG介导的对PMN功能的抑制作用。用3.12微摩尔或更高浓度的SANG处理PMN也导致PMN脱颗粒和吞噬作用受到抑制。结果表明，SANG介导的对PMN功能的抑制作用，无细胞溶解或炎性介质的释放，可能具有临床意义。
