Xue Dan, Yin Hongxiang, Li Jie, Liu Xianbo, Zhang Hao, Peng Cheng
Key Laboratory of Drug Targeting and Drug Delivery System, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
Microsc Res Tech. 2009 Oct;72(10):744-54. doi: 10.1002/jemt.20726.
In the trade and raw drug market, the medicinal plants of genus Paris are available in the form of rhizomes without any vouchers, thus making it difficult to identify and distinguish the different species of Genus Paris. Recent studies have shown that the species of Paris possess different chemical constituents, pharmacological activities, and efficiencies in clinical application. To distinguish 11 species of Paris collected from the western Sichuan province of China and ensure their safety and efficacy, in the present work, the microscopic characteristics of rhizomes and the crude drug powder of the 11 species of Paris were compared using a light microscope according to the usual microscopic techniques. The results of the microscopic features were systematically described and illustrated. The differences among these species are great enough that the identity of most material can be easily determined. Also, semiquantitative and quantitative micrographic parameter tables were simultaneously presented. Further, the key authentication parameters based on these anatomic characteristics analyzed was drawn up and presented for the Paris species studied. The study indicated that light microscopy and related techniques could provide a method that is convenient, feasible, and can be unambiguously applied in the authentication of species of Paris. This information is required not only for the identification procedures that guarantee the utilization of the appropriate raw material, but also for the quality control standards demanded.
在贸易和生药市场中，重楼属药用植物以根茎形式出售，且无任何凭证，因此难以鉴别和区分重楼属的不同物种。最近的研究表明，重楼属不同物种具有不同的化学成分、药理活性及临床应用功效。为鉴别采自中国四川省西部的11种重楼并确保其安全性和有效性，本研究按照常规显微技术，使用光学显微镜比较了这11种重楼根茎及生药粉末的显微特征。对显微特征结果进行了系统描述和图示说明。这些物种间的差异足够大，以至于大多数材料的身份都能轻易确定。同时还给出了半定量和定量显微图像参数表。此外，根据所分析的这些解剖学特征制定并给出了所研究重楼物种的关键鉴定参数。研究表明，光学显微镜及相关技术可为重楼属物种鉴定提供一种方便、可行且能明确应用的方法。这些信息不仅是保证使用合适原材料的鉴定程序所必需的，也是质量控制标准所要求的。
