一种用于测定功能性和感染性腺病毒滴度的细胞培养与定量聚合酶链反应整合技术。

An integrated cell culture and quantitative polymerase chain reaction technique for determining titers of functional and infectious adenoviruses.

作者信息

Li Feng, Feng Liqiang, Liu Yichu, Zhen Xuehua, Chen Ling

机构信息

Center for Vaccines and Biotherapeutics, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou International Business Incubator A3, Guangzhou Science City, Guangzhou 510663, China.

出版信息

Anal Biochem. 2009 Aug 15;391(2):157-9. doi: 10.1016/j.ab.2009.05.017. Epub 2009 May 18.

DOI:10.1016/j.ab.2009.05.017

PMID:19457433

Abstract

We describe a simple and rapid assay for determining the titer of functional and infectious adenoviruses that yields reliable results within 36 h. The method consists of an initial incubation of serial diluted adenoviruses on HEK293 cells and a subsequent detection of adenovirus genomic DNA with quantitative polymerase chain reaction. With an adenovirus of known titer as a reference, the exact titers of four unknown adenoviruses could be easily and accurately determined in one 96-well plate. This method can be potentially modified for quality control of other viruses.

摘要

我们描述了一种简单快速的检测方法，用于测定功能性和感染性腺病毒的滴度，该方法可在36小时内得出可靠结果。该方法包括将系列稀释的腺病毒首先在HEK293细胞上孵育，随后用定量聚合酶链反应检测腺病毒基因组DNA。以已知滴度的腺病毒作为参考，在一个96孔板中可以轻松准确地测定四种未知腺病毒的准确滴度。该方法可能会被改进用于其他病毒的质量控制。