细颗粒物（PM10）对人肺成纤维细胞的影响。
The effect of PM10 on human lung fibroblasts.
作者信息
Alley Df, Langley-Turnbaugh S, Gordon Nr, Wise Jp, Van Epps G, Jalbert A
机构信息
Department of Chemistry, University of Southern Maine, Gorham, Maine, USA.
出版信息
Toxicol Ind Health. 2009 Mar;25(2):111-20. doi: 10.1177/0748233709103185.
Asthma diagnoses are increasing nationally with the highest rates in the New England states. Epidemiological studies have suggested a relationship between airborne particulate matter (PM) and severity of an asthma attack. However, because particulate matter, PM, is such a complex mixture, it is difficult to isolate the exacerbating factors. In this paper we investigate the effect of NIST (National Institute of Standards and Technology) and Maine PM and the soluble metals released from the PM on the growth of human lung fibroblasts. While the NIST PM itself had the most pronounced effect on cell survival rates, solutions of metals extracted from the PM also affected cell survival. Treatment of cells with 10, 50, 100 and 200 ug/cm(2) resulted in 84 +/- 13%, 69 +/- 15%, 58 +/- 14% and 58 +/- 16% survival, respectively. Appropriate concentrations of eight acid soluble metals from NIST PM were determined and tested on cells giving 91 +/- 11%, 87 +/- 10%, 72 +/- 18% and 66 +/- 20% survival, respectively. Soluble metals from Maine PM were extracted and mixtures of appropriate concentrations of these metals were used to treat cells, resulting in 88 +/- 5%, 81 +/- 5%, 79 +/- 3% and 57 +/- 9% survival rate. To determine which, if any, of the metals individually affected the cells, Mn, Cu, V and As were used to treat the cells. At the metal concentrations tested, only As and V affected cell survival.
哮喘诊断在全国范围内呈上升趋势，新英格兰各州的发病率最高。流行病学研究表明，空气中的颗粒物（PM）与哮喘发作的严重程度之间存在关联。然而，由于颗粒物是一种非常复杂的混合物，很难分离出加重病情的因素。在本文中，我们研究了美国国家标准与技术研究院（NIST）的颗粒物和缅因州的颗粒物以及颗粒物释放的可溶性金属对人肺成纤维细胞生长的影响。虽然NIST颗粒物本身对细胞存活率的影响最为显著，但从颗粒物中提取的金属溶液也会影响细胞存活。用10、50、100和200微克/平方厘米处理细胞后，细胞存活率分别为84±13%、69±15%、58±14%和58±16%。确定了NIST颗粒物中八种酸溶性金属的合适浓度，并对细胞进行测试，细胞存活率分别为91±11%、87±10%、72±18%和66±20%。提取了缅因州颗粒物中的可溶性金属，并使用这些金属的合适浓度混合物处理细胞，细胞存活率分别为88±5%、81±5%、79±3%和57±9%。为了确定哪些金属（如果有的话）会单独影响细胞，使用锰、铜、钒和砷处理细胞。在所测试的金属浓度下，只有砷和钒影响细胞存活。
Zotero 插件