Fang G W, Cech T R
Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder 80309.
Nucleic Acids Res. 1991 Oct 25;19(20):5515-8. doi: 10.1093/nar/19.20.5515.
A telomere-binding protein heterodimer of 56 kDa (alpha) and 41 kDa (beta) subunits binds specifically to Oxytricha nova telomeres. Genes encoding both subunits have been cloned previously. Here we report molecular cloning and sequence analysis of the homologous genes in Stylonychia mytilis. The derived amino acid sequences were 79% identical for the alpha subunits and 77% identical for the beta subunits. Three repeats of a Leu/Ile heptad were found in each subunit, which might be involved in heterodimer formation. A 360 amino acid region of the Stylonychia mytilis alpha subunit was found to share weak sequence similarity with human vimentin, suggesting the possibility of a relationship between telomeres and intermediate filaments.
一种由56 kDa（α）和41 kDa（β）亚基组成的端粒结合蛋白异二聚体可特异性结合新大草履虫的端粒。此前已克隆出编码这两个亚基的基因。在此，我们报告了贻贝棘尾虫中同源基因的分子克隆及序列分析。推导的α亚基氨基酸序列一致性为79%，β亚基为77%。在每个亚基中发现了三个亮氨酸/异亮氨酸七肽重复序列，这可能参与异二聚体的形成。发现贻贝棘尾虫α亚基的一个360个氨基酸的区域与人波形蛋白具有较弱的序列相似性，这表明端粒与中间丝之间可能存在关联。
